10.3389/fmicb.2018.02268.s001
Jaewook Lee
Jaewook
Lee
Yae Jin Yoon
Yae
Jin Yoon
Ji Hyun Kim
Ji Hyun
Kim
Nhung Thi Hong Dinh
Nhung Thi Hong
Dinh
Gyeongyun Go
Gyeongyun
Go
Sookil Tae
Sookil
Tae
Kyong-Su Park
Kyong-Su
Park
Hyun Taek Park
Hyun
Taek Park
Changjin Lee
Changjin
Lee
Tae-Young Roh
Tae-Young
Roh
Dolores Di Vizio
Dolores Di
Vizio
Yong Song Gho
Yong Song
Gho
Table_1_Outer Membrane Vesicles Derived From Escherichia coli Regulate Neutrophil Migration by Induction of Endothelial IL-8.DOCX
Frontiers
2018
outer membrane vesicles
extracellular vesicles
exosomes
neutrophil
pulmonary inflammation
IL-8
NF-κB
toll-like receptor 4
2018-10-11 04:06:30
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Outer_Membrane_Vesicles_Derived_From_Escherichia_coli_Regulate_Neutrophil_Migration_by_Induction_of_Endothelial_IL-8_DOCX/7194524
<p>Outer membrane vesicles (OMVs) are spherical, proteolipid nanostructures that are constitutively released by Gram-negative bacteria including Escherichia coli. Although it has been shown that administration of E. coli OMVs stimulates a strong pulmonary inflammatory response with infiltration of neutrophils into the lungs in vivo, the mechanism of E. coli OMV-mediated neutrophil recruitment is poorly characterized. In this study, we observed significant infiltration of neutrophils into the mouse lung tissues in vivo, with increased expression of the neutrophil chemoattractant CXCL1, a murine functional homolog of human IL-8, on intraperitoneal administration of E. coli OMVs. In addition, OMVs and CD31-positive endothelial cells colocalized in the mouse lungs. Moreover, in vitro results showed that E. coli OMVs significantly increased IL-8 release from human microvascular endothelial cells and toll-like receptor (TLR)4 was found to be the main component for recognizing E. coli OMVs among human endothelial cell-associated TLRs. Furthermore, the transmigration of neutrophils was suppressed in the lung tissues obtained from TLR4 knockout mice treated with E. coli OMVs. Taken together, our data demonstrated that E. coli OMVs potently recruit neutrophils into the lung via the release of IL-8/CXCL1 from endothelial cells in TLR4- and NF-κB-dependent manners.</p>