10.3389/fphys.2018.01348.s001
Elizabeth Chen
Elizabeth
Chen
Dennis Kolosov
Dennis
Kolosov
Michael J. O'Donnell
Michael J.
O'Donnell
Martin A. Erlandson
Martin A.
Erlandson
Jeremy N. McNeil
Jeremy
N. McNeil
Cam Donly
Cam
Donly
Table_1_The Effect of Diet on Midgut and Resulting Changes in Infectiousness of AcMNPV Baculovirus in the Cabbage Looper, Trichoplusia ni.XLSX
Frontiers
2018
cabbage looper
AcMNPV baculovirus
chitinase
chitin deacetylase
midgut transcriptome
pathogen resistance
peritrophic matrix
midgut pH
2018-10-04 04:15:05
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_The_Effect_of_Diet_on_Midgut_and_Resulting_Changes_in_Infectiousness_of_AcMNPV_Baculovirus_in_the_Cabbage_Looper_Trichoplusia_ni_XLSX/7163888
<p>Insecticide resistance has been reported in many important agricultural pests, and alternative management methods are required. Baculoviruses qualify as an effective, yet environmentally benign, biocontrol agent but their efficacy against generalist herbivores may be influenced by diet. However, few studies have investigated the tritrophic interactions of plant, pest, and pathogen from both a gene expression and physiological perspective. Here we use microscopy and transcriptomics to examine how diet affects the structure of peritrophic matrix (PM) in Trichoplusia ni larvae and consequently their susceptibility to the baculovirus, AcMNPV. Larvae raised on potato leaves had lower transcript levels for chitinase and chitin deacetylase genes, and possessed a thicker and more multi-layered PM than those raised on cabbage or artificial diet, which could contribute to their significantly lower susceptibility to the baculovirus. The consequences of these changes underline the importance of considering dietary influences on pathogen susceptibility in pest management strategies.</p>