10.3389/fimmu.2018.02196.s001
Arata Takeuchi
Arata
Takeuchi
Madoka Ozawa
Madoka
Ozawa
Yasuhiro Kanda
Yasuhiro
Kanda
Mina Kozai
Mina
Kozai
Izumi Ohigashi
Izumi
Ohigashi
Yoichi Kurosawa
Yoichi
Kurosawa
Md Azizur Rahman
Md Azizur
Rahman
Toshihiko Kawamura
Toshihiko
Kawamura
Yuto Shichida
Yuto
Shichida
Eiji Umemoto
Eiji
Umemoto
Masayuki Miyasaka
Masayuki
Miyasaka
Burkhard Ludewig
Burkhard
Ludewig
Yousuke Takahama
Yousuke
Takahama
Takashi Nagasawa
Takashi
Nagasawa
Tomoya Katakai
Tomoya
Katakai
Video_1_A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node.MOV
Frontiers
2018
chemokines
deep cortex
fibroblastic stromal cells
lymph node
medulla
subcompartment
2018-10-02 04:45:43
Media
https://frontiersin.figshare.com/articles/media/Video_1_A_Distinct_Subset_of_Fibroblastic_Stromal_Cells_Constitutes_the_Cortex-Medulla_Boundary_Subcompartment_of_the_Lymph_Node_MOV/7156187
<p>The spatiotemporal regulation of immune responses in the lymph node (LN) depends on its sophisticated tissue architecture, consisting of several subcompartments supported by distinct fibroblastic stromal cells (FSCs). However, the intricate details of stromal structures and associated FSC subsets are not fully understood. Using several gene reporter mice, we sought to discover unrecognized stromal structures and FSCs in the LN. The four previously identified FSC subsets in the cortex are clearly distinguished by the expression pattern of reporters including PDGFRβ, CCL21-ser, and CXCL12. Herein, we identified a unique FSC subset expressing both CCL21-ser and CXCL12 in the deep cortex periphery (DCP) that is characterized by preferential B cell localization. This subset was clearly different from CXCL12<sup>high</sup>LepR<sup>high</sup> FSCs in the medullary cord, which harbors plasma cells. B cell localization in the DCP was controlled chiefly by CCL21-ser and, to a lesser extent, CXCL12. Moreover, the optimal development of the DCP as well as medulla requires B cells. Together, our findings suggest the presence of a unique microenvironment in the cortex-medulla boundary and offer an advanced view of the multi-layered stromal framework constructed by distinct FSC subsets in the LN.</p>