10.3389/fimmu.2018.02196.s001 Arata Takeuchi Arata Takeuchi Madoka Ozawa Madoka Ozawa Yasuhiro Kanda Yasuhiro Kanda Mina Kozai Mina Kozai Izumi Ohigashi Izumi Ohigashi Yoichi Kurosawa Yoichi Kurosawa Md Azizur Rahman Md Azizur Rahman Toshihiko Kawamura Toshihiko Kawamura Yuto Shichida Yuto Shichida Eiji Umemoto Eiji Umemoto Masayuki Miyasaka Masayuki Miyasaka Burkhard Ludewig Burkhard Ludewig Yousuke Takahama Yousuke Takahama Takashi Nagasawa Takashi Nagasawa Tomoya Katakai Tomoya Katakai Video_1_A Distinct Subset of Fibroblastic Stromal Cells Constitutes the Cortex-Medulla Boundary Subcompartment of the Lymph Node.MOV Frontiers 2018 chemokines deep cortex fibroblastic stromal cells lymph node medulla subcompartment 2018-10-02 04:45:43 Media https://frontiersin.figshare.com/articles/media/Video_1_A_Distinct_Subset_of_Fibroblastic_Stromal_Cells_Constitutes_the_Cortex-Medulla_Boundary_Subcompartment_of_the_Lymph_Node_MOV/7156187 <p>The spatiotemporal regulation of immune responses in the lymph node (LN) depends on its sophisticated tissue architecture, consisting of several subcompartments supported by distinct fibroblastic stromal cells (FSCs). However, the intricate details of stromal structures and associated FSC subsets are not fully understood. Using several gene reporter mice, we sought to discover unrecognized stromal structures and FSCs in the LN. The four previously identified FSC subsets in the cortex are clearly distinguished by the expression pattern of reporters including PDGFRβ, CCL21-ser, and CXCL12. Herein, we identified a unique FSC subset expressing both CCL21-ser and CXCL12 in the deep cortex periphery (DCP) that is characterized by preferential B cell localization. This subset was clearly different from CXCL12<sup>high</sup>LepR<sup>high</sup> FSCs in the medullary cord, which harbors plasma cells. B cell localization in the DCP was controlled chiefly by CCL21-ser and, to a lesser extent, CXCL12. Moreover, the optimal development of the DCP as well as medulla requires B cells. Together, our findings suggest the presence of a unique microenvironment in the cortex-medulla boundary and offer an advanced view of the multi-layered stromal framework constructed by distinct FSC subsets in the LN.</p>