10.3389/fimmu.2018.02225.s001
Mei Qiu Lim
Mei Qiu
Lim
Emmanuelle A. P. Kumaran
Emmanuelle A. P.
Kumaran
Hwee Cheng Tan
Hwee
Cheng Tan
David C. Lye
David
C. Lye
Yee Sin Leo
Yee
Sin Leo
Eng Eong Ooi
Eng
Eong Ooi
Paul A. MacAry
Paul
A. MacAry
Antonio Bertoletti
Antonio
Bertoletti
Laura Rivino
Laura
Rivino
Data_Sheet_1_Cross-Reactivity and Anti-viral Function of Dengue Capsid and NS3-Specific Memory T Cells Toward Zika Virus.PDF
Frontiers
2018
dengue virus (DENV)
Zika virus (ZIKV)
T cells
cross-reactive immune response
heterologous immunity
anti-viral response
2018-10-01 13:53:59
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Cross-Reactivity_and_Anti-viral_Function_of_Dengue_Capsid_and_NS3-Specific_Memory_T_Cells_Toward_Zika_Virus_PDF/7152260
<p>Zika virus (ZIKV), a flavivirus with homology to dengue virus (DENV), is spreading to areas of DENV hyper-endemicity. Heterologous T cell immunity, whereby virus-specific memory T cells are activated by variant peptides derived from a different virus, can lead to enhanced viral clearance or diminished protective immunity and altered immunopathology. In mice, CD8+ T cells specific for DENV provide in vivo protective efficacy against subsequent ZIKV infection. In humans, contrasting studies report complete absence or varying degrees of DENV/ZIKV T cell cross-reactivity. Moreover, the impact of cross-reactive T cell recognition on the anti-viral capacity of T cells remains unclear. Here, we show that DENV-specific memory T cells display robust cross-reactive recognition of ZIKV NS3 ex vivo and after in vitro expansion in respectively n = 7/10 and n = 9/9 dengue-immune individuals tested. In contrast, cross-reactivity toward ZIKV capsid is low or absent. Cross-reactive recognition of DENV or ZIKV NS3 peptides elicits similar production of the anti-viral effector mediators IFN-γ, TNF-α, and CD107a. We identify 9 DENV/ZIKV cross-reactive epitopes, 7 of which are CD4+ and 2 are CD8+ T cell epitopes. We also show that cross-reactive CD4+ and CD8+ T cells targeting novel NS3 epitopes display anti-viral effector potential toward ZIKV-infected cells, with CD8+ T cells mediating direct lyses of these cells. Our results demonstrate that DENV NS3-specific memory T cells display anti-viral effector capacity toward ZIKV, suggesting a potential beneficial effect in humans of pre-existing T cell immunity to DENV upon ZIKV infection.</p>