10.3389/fphar.2018.01053.s001
Jiahong Meng
Jiahong
Meng
Chenhe Zhou
Chenhe
Zhou
Bin Hu
Bin
Hu
Mengmeng Luo
Mengmeng
Luo
Yute Yang
Yute
Yang
Yangxin Wang
Yangxin
Wang
Wei Wang
Wei
Wang
Guangyao Jiang
Guangyao
Jiang
Jianqiao Hong
Jianqiao
Hong
Sihao Li
Sihao
Li
Haobo Wu
Haobo
Wu
Shigui Yan
Shigui
Yan
Weiqi Yan
Weiqi
Yan
Image_1_Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation.TIF
Frontiers
2018
aseptic loosening
osteoclast
NF-κB – nuclear factor-kappa B
MAPK
TAK1
2018-09-26 13:30:21
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Stevioside_Prevents_Wear_Particle-Induced_Osteolysis_by_Inhibiting_Osteoclastogenesis_and_Inflammatory_Response_via_the_Suppression_of_TAK1_Activation_TIF/7133642
<p>Aseptic loosening and periprosthetic osteolysis are the leading causes of total joint arthroplasty failure, which occurs as a result of chronic inflammatory response and enhanced osteoclast activity. Here we showed that stevioside, a natural compound isolated from Stevia rebaudiana, exhibited preventative effects on titanium particle-induced osteolysis in a mouse calvarial model. Further histological assessment and real-time PCR analysis indicated that stevioside prevented titanium particle-induced osteolysis by inhibiting osteoclast formation and inflammatory cytokine expression in vivo. In vitro, we found that stevioside could suppress RANKL-induced osteoclastogenesis and titanium particle-induced inflammatory response in a dose-dependent manner. Mechanistically, stevioside achieved these effects by disrupting the phosphorylation of TAK1 and subsequent activation of NF-κB/MAPKs signaling pathways. Collectively, our data suggest that stevioside effectively suppresses osteoclastogenesis and inflammatory response both in vitro and in vivo, and it might be a potential therapy for particle-induced osteolysis and other osteolytic diseases.</p>