%0 Figure %A Takahashi, Shinya %A Sakamaki, Midori %A Ferdousi, Farhana %A Yoshida, Masaki %A Demura, Mikihide %A M. Watanabe, Makoto %A Isoda, Hiroko %D 2018 %T Image_1_Ethanol Extract of Aurantiochytrium mangrovei 18W-13a Strain Possesses Anti-inflammatory Effects on Murine Macrophage RAW264 Cells.tif %U https://frontiersin.figshare.com/articles/figure/Image_1_Ethanol_Extract_of_Aurantiochytrium_mangrovei_18W-13a_Strain_Possesses_Anti-inflammatory_Effects_on_Murine_Macrophage_RAW264_Cells_tif/7131956 %R 10.3389/fphys.2018.01205.s001 %2 https://frontiersin.figshare.com/ndownloader/files/13122149 %K anti-inflammation %K Aurantiochytrium %K microalgae %K RAW264 cells %K pro-inflammatory cytokines %K nitric oxide %K lipopolysaccharide %X

In this study, the effects of an ethanolic extract of Aurantiochytrium mangrovei 18W-13a strain (AM18W-13a) on lipopolysaccharide (LPS)-induced inflammatory responses in RAW264 murine macrophages were studied. Pre-treatment with the AM18W-13a extract significantly suppressed the LPS-induced production of nitric oxide and pro-inflammatory cytokines. RAW264 cells treated with the AM18W-13a extract for 1 and 24 h were subjected to DNA microarray analyses for detecting the differentially expressed genes. The treatment of RAW264 cells with the AM18W-13a extract for 24 h significantly suppressed the expression of several genes associated with inflammation or chemotaxis. Furthermore, treatment with the AM18W-13a extract for 1 h suppressed the expression of Pde4b, but induced the expression of Egr2 and Egr3 in RAW264 cells. Additionally, the AM18W-13a extract significantly enhanced the expression of certain anti-inflammatory mediators. This study is the first report of the anti-inflammatory effects of the AM18W-13a extract and its mechanism of action in LPS-stimulated murine macrophages.

%I Frontiers