10.3389/fimmu.2018.02158.s001
Mykolas Bendorius
Mykolas
Bendorius
Indira Neeli
Indira
Neeli
Fengjuan Wang
Fengjuan
Wang
Srinivasa Reddy Bonam
Srinivasa Reddy
Bonam
Eszter Dombi
Eszter
Dombi
Nelly Buron
Nelly
Buron
Annie Borgne-Sanchez
Annie
Borgne-Sanchez
Joanna Poulton
Joanna
Poulton
Marko Radic
Marko
Radic
Sylviane Muller
Sylviane
Muller
Data_Sheet_1_The Mitochondrion-lysosome Axis in Adaptive and Innate Immunity: Effect of Lupus Regulator Peptide P140 on Mitochondria Autophagy and NETosis.PDF
Frontiers
2018
NETosis
autophagy
mitochondrion
systemic lupus erythematosus
neuroinflammation
P140 peptide
2018-09-26 04:12:27
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_The_Mitochondrion-lysosome_Axis_in_Adaptive_and_Innate_Immunity_Effect_of_Lupus_Regulator_Peptide_P140_on_Mitochondria_Autophagy_and_NETosis_PDF/7131950
<p>Mitochondria deserve special attention as sensors of cellular energy homeostasis and metabolic state. Moreover, mitochondria integrate intra- and extra-cellular signals to determine appropriate cellular responses that range from proliferation to cell death. In autoimmunity, as in other inflammatory chronic disorders, the metabolism of immune cells may be extensively remodeled, perturbing sensitive tolerogenic mechanisms. Here, we examine the distribution and effects of the therapeutic 21-mer peptide called P140, which shows remarkable efficacy in modulating immune responses in inflammatory settings. We measured P140 and control peptide effects on isolated mitochondria, the distribution of peptides in live cells, and their influence on the levels of key autophagy regulators. Our data indicate that while P140 targets macro- and chaperone-mediated autophagy processes, it has little effect, if any, on mitochondrial autophagy. Remarkably, however, it suppresses NET release from neutrophils exposed to immobilized NET-anti-DNA IgG complexes. Together, our results suggest that in the mitochondrion-lysosome axis, a likely driver of NETosis and inflammation, the P140 peptide does not operate by affecting mitochondria directly.</p>