10.3389/fimmu.2018.02044.s002 Zachary L. Benet Zachary L. Benet Matangi Marthi Matangi Marthi Fang Ke Fang Ke Rita Wu Rita Wu Jackson S. Turner Jackson S. Turner Jahan B. Gabayre Jahan B. Gabayre Michael I. Ivanitskiy Michael I. Ivanitskiy Sahil S. Sethi Sahil S. Sethi Irina L. Grigorova Irina L. Grigorova Video_1_CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes.MP4 Frontiers 2018 germinal centers follicular regulatory T cells (Tfr) CCL3/MIP1-alpha 2-photon imaging follicular helper T cells 2018-09-13 04:13:45 Media https://frontiersin.figshare.com/articles/media/Video_1_CCL3_Promotes_Germinal_Center_B_Cells_Sampling_by_Follicular_Regulatory_T_Cells_in_Murine_Lymph_Nodes_MP4/7081448 <p>Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.</p>