10.3389/fimmu.2018.02044.s002
Zachary L. Benet
Zachary L.
Benet
Matangi Marthi
Matangi
Marthi
Fang Ke
Fang
Ke
Rita Wu
Rita
Wu
Jackson S. Turner
Jackson S.
Turner
Jahan B. Gabayre
Jahan B.
Gabayre
Michael I. Ivanitskiy
Michael I.
Ivanitskiy
Sahil S. Sethi
Sahil S.
Sethi
Irina L. Grigorova
Irina L.
Grigorova
Video_1_CCL3 Promotes Germinal Center B Cells Sampling by Follicular Regulatory T Cells in Murine Lymph Nodes.MP4
Frontiers
2018
germinal centers
follicular regulatory T cells (Tfr)
CCL3/MIP1-alpha
2-photon imaging
follicular helper T cells
2018-09-13 04:13:45
Media
https://frontiersin.figshare.com/articles/media/Video_1_CCL3_Promotes_Germinal_Center_B_Cells_Sampling_by_Follicular_Regulatory_T_Cells_in_Murine_Lymph_Nodes_MP4/7081448
<p>Previous studies and our findings suggest upregulated expression of proinflammatory chemokines CCL3/4 in germinal center (GC) centrocytes. However, the role of CCL3/4 for centrocyte interactions with follicular T cells and regulation of humoral immunity is poorly understood. We found that CCL3 promotes chemotaxis of Tfr cells ex vivo. Two-photon imaging revealed that B cells-intrinsic production of CCL3 promotes their probing by follicular regulatory T cells (Tfr) within GCs of murine lymph nodes. Overall this study suggests that CCL3 facilitates direct interactions of foreign antigen-specific GC B cells and their negative regulation with Tfr cells in vivo.</p>