10.3389/fcimb.2018.00281.s002 Mireia Lopez-Siles Mireia Lopez-Siles Núria Enrich-Capó Núria Enrich-Capó Xavier Aldeguer Xavier Aldeguer Miriam Sabat-Mir Miriam Sabat-Mir Sylvia H. Duncan Sylvia H. Duncan L. Jesús Garcia-Gil L. Jesús Garcia-Gil Margarita Martinez-Medina Margarita Martinez-Medina Image_2_Alterations in the Abundance and Co-occurrence of Akkermansia muciniphila and Faecalibacterium prausnitzii in the Colonic Mucosa of Inflammatory Bowel Disease Subjects.pdf Frontiers 2018 Akkermansia muciniphila Faecalibacterium prausnitzii Crohn's disease ulcerative colitis inflammatory bowel diseases 2018-09-07 13:57:03 Figure https://frontiersin.figshare.com/articles/figure/Image_2_Alterations_in_the_Abundance_and_Co-occurrence_of_Akkermansia_muciniphila_and_Faecalibacterium_prausnitzii_in_the_Colonic_Mucosa_of_Inflammatory_Bowel_Disease_Subjects_pdf/7059050 <p>Akkermansia muciniphila and Faecalibacterium prausnitzii, cohabitants in the intestinal mucosa, are considered members of a healthy microbiota and reduction of both species occurs in several intestinal disorders, including inflammatory bowel disease. Little is known however about a possible link between the reduction in quantity of these species, and in which circumstances this may occur. This study aims to determine the abundances and co-occurrence of the two species in order to elucidate conditions that may compromise their presence in the gut. Loads of A. muciniphila, total F. prausnitzii and its two phylogroup (16S rRNA gene copies) were determined by quantitative polymerase chain reaction in colonic biopsies from 17 healthy controls (H), 23 patients with ulcerative colitis (UC), 31 patients with Crohn's disease (CD), 3 with irritable bowel syndrome (IBS) and 3 with colorectal cancer (CRC). Data were normalized to total bacterial 16S rRNA gene copies in the same sample. Prevalence, relative abundances and correlation analyses were performed according to type of disease and considering relevant clinical characteristics of patients such as IBD location, age of disease onset, CD behavior, current medication and activity status. Co-occurrence of both species was found in 29% of H, 65% of UC and 29% of CD. Lower levels of total F. prausnitzii and phylogroups were found in subjects with CD, compared with H subjects (P ≤ 0.044). In contrast, no differences were found with the regard to A. muciniphila abundance across different disease states, but CD patients with disease onset below 16 years of age featured a marked depletion of this species. In CD patients, correlation between A. muciniphila and total F. prausnitzii (ρ = 0.362, P = 0.045) was observed, and particularly in those with non-stricturing, non-penetrating disease behavior and under moderate immunosuppressants therapy. Altogether, this study revealed that co-occurrence of both species differs between disease status. In addition, IBD patients featured a reduction of F. prausnitzii but similar loads of A. muciniphila when compared to H subjects, with the exception of those with early onset CD. Depletion of A. muciniphila in this subgroup of subjects suggests that it could be a potential biomarker to assist in pediatric CD diagnosis.</p>