10.3389/fnmol.2018.00309.s001
Nadine Kerr
Nadine
Kerr
Marta García-Contreras
Marta
García-Contreras
Sam Abbassi
Sam
Abbassi
Nancy H. Mejias
Nancy H.
Mejias
Brandon R. Desousa
Brandon R.
Desousa
Camillo Ricordi
Camillo
Ricordi
W. Dalton Dietrich
W.
Dalton Dietrich
Robert W. Keane
Robert
W. Keane
Juan Pablo de Rivero Vaccari
Juan
Pablo de Rivero Vaccari
Image_1_Inflammasome Proteins in Serum and Serum-Derived Extracellular Vesicles as Biomarkers of Stroke.JPEG
Frontiers
2018
inflammasome
biomarkers
extracellular vesicles
serum
exosomes
caspase-1
ASC
2018-09-04 07:59:15
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Inflammasome_Proteins_in_Serum_and_Serum-Derived_Extracellular_Vesicles_as_Biomarkers_of_Stroke_JPEG/7041833
<p>The inflammasome is a key contributor to the inflammatory innate immune response after stroke. We have previously shown that inflammasome proteins are released in extracellular vesicles (EV) after brain and spinal cord injury. In addition, we have shown that inflammasome proteins offer great promise as biomarkers of central nervous system (CNS) injury following brain trauma. In the present study, we used a Simple Plex Assay (Protein Simple), a novel multi-analyte automated microfluidic immunoassay platform, to analyze serum and serum-derived EV samples from stroke patients and control subjects for inflammasome protein levels of caspase-1, apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC), Interleukins (IL)-1β, and (IL)-18. Receiver operator characteristic (ROC) curves with associated confidence intervals obtained from the analysis of serum samples revealed that the area under the curve (AUC) for ASC was 0.99 with a confidence interval between 0.9914 and 1.004, whereas the AUC for caspase-1, IL-1β, and IL-18 were 0.75, 0.61, and 0.67, respectively. Thus, these data indicate that ASC is a potential biomarker of stroke and highlight the role of the inflammasome in the inflammatory response after brain ischemia.</p>