10.3389/fmicb.2018.01828.s001
Ling Liu
Ling
Liu
Qiaochu Wang
Qiaochu
Wang
Ying Sun
Ying
Sun
Yanhua Zhang
Yanhua
Zhang
Xianghui Zhang
Xianghui
Zhang
Jinliang Liu
Jinliang
Liu
Gang Yu
Gang
Yu
Hongyu Pan
Hongyu
Pan
Presentation_1_Sssfh1, a Gene Encoding a Putative Component of the RSC Chromatin Remodeling Complex, Is Involved in Hyphal Growth, Reactive Oxygen Species Accumulation, and Pathogenicity in Sclerotinia sclerotiorum.pdf
Frontiers
2018
RNA interference
Sclerotinia sclerotiorum
SsSFH1
SsMSG5
ROS accumulation
pathogenicity
2018-08-07 04:20:43
Presentation
https://frontiersin.figshare.com/articles/presentation/Presentation_1_Sssfh1_a_Gene_Encoding_a_Putative_Component_of_the_RSC_Chromatin_Remodeling_Complex_Is_Involved_in_Hyphal_Growth_Reactive_Oxygen_Species_Accumulation_and_Pathogenicity_in_Sclerotinia_sclerotiorum_pdf/6939659
<p>SFH1 (for Snf5 homolog) protein, comprised in the RSC (Remodels Structure of Chromatin) chromatin remodeling complex, functions as a transcription factor (TF) to specifically regulate gene transcription and chromatin remodeling. As one of the well-conserved TFs in eukaryotic organisms, little is known about the roles of SFH1 protein in the filamentous fungi. In Sclerotinia sclerotiorum, one of the notorious plant fungal pathogens, there are nine proteins predicted to contain GATA-box domain according to GATA family TF classification, among which Sssfh1 (SS1G_01151) encodes a protein including a GATA-box domain and a SNF5 domain. Here, we characterized the roles of Sssfh1 in the developmental process and fungal pathogenicity by using RNA interference (RNAi)-based gene silencing in S. sclerotiorum. RNA-silenced strains with significantly reduced Sssfh1 RNA levels exhibited slower hyphal growth and decreased reactive oxygen species (ROS) accumulation in hyphae compared to the wild-type (WT) strain. Yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays demonstrated that SsSFH1 interacts with SsMSG5, a MAPK phosphatase in S. sclerotiorum. Furthermore, Sssfh1-silenced strains exhibited enhanced tolerance to NaCl and H<sub>2</sub>O<sub>2</sub>. Results of infection assays on soybean and common bean (Phaseolus vulgaris) leaves indicated that Sssfh1 is required for full virulence of S. sclerotiorum during infection in the susceptible host plants. Collectively, our results suggest that the TF SsSFH1 is involved in growth, ROS accumulation and virulence in S. sclerotiorum.</p>