Pallarès, Irantzu S. de Groot, Natalia Iglesias, Valentín Sant’Anna, Ricardo Biosca, Arnau Fernàndez-Busquets, Xavier Ventura, Salvador Table_1_Discovering Putative Prion-Like Proteins in Plasmodium falciparum: A Computational and Experimental Analysis.XLSX <p>Prions are a singular subset of proteins able to switch between a soluble conformation and a self-perpetuating amyloid state. Traditionally associated with neurodegenerative diseases, increasing evidence indicates that organisms exploit prion-like mechanisms for beneficial purposes. The ability to transit between conformations is encoded in the so-called prion domains, long disordered regions usually enriched in glutamine/asparagine residues. Interestingly, Plasmodium falciparum, the parasite that causes the most virulent form of malaria, is exceptionally rich in proteins bearing long Q/N-rich sequence stretches, accounting for roughly 30% of the proteome. This biased composition suggests that these protein regions might correspond to prion-like domains (PrLDs) and potentially form amyloid assemblies. To investigate this possibility, we performed a stringent computational survey for Q/N-rich PrLDs on P. falciparum. Our data indicate that ∼10% of P. falciparum protein sequences have prionic signatures, and that this subproteome is enriched in regulatory proteins, such as transcription factors and RNA-binding proteins. Furthermore, we experimentally demonstrate for several of the identified PrLDs that, despite their disordered nature, they contain inner short sequences able to spontaneously self-assemble into amyloid-like structures. Although the ability of these sequences to nucleate the conformational conversion of the respective full-length proteins should still be demonstrated, our analysis suggests that, as previously described for other organisms, prion-like proteins might also play a functional role in P. falciparum.</p> Plasmodium;protein aggregation;amyloid;prion;Q/N-rich sequences;protein disorder 2018-08-07
    https://frontiersin.figshare.com/articles/dataset/Table_1_Discovering_Putative_Prion-Like_Proteins_in_Plasmodium_falciparum_A_Computational_and_Experimental_Analysis_XLSX/6939545
10.3389/fmicb.2018.01737.s003