10.3389/fmicb.2018.01697.s004
Jeremy R. Chen See
Jeremy R. Chen
See
Nikea Ulrich
Nikea
Ulrich
Hephzibah Nwanosike
Hephzibah
Nwanosike
Christopher J. McLimans
Christopher J.
McLimans
Vasily Tokarev
Vasily
Tokarev
Justin R. Wright
Justin R.
Wright
Maria F. Campa
Maria F.
Campa
Christopher J. Grant
Christopher J.
Grant
Terry C. Hazen
Terry C.
Hazen
Jonathan M. Niles
Jonathan M.
Niles
Daniel Ressler
Daniel
Ressler
Regina Lamendella
Regina
Lamendella
Table_3_Bacterial Biomarkers of Marcellus Shale Activity in Pennsylvania.XLSX
Frontiers
2018
hydraulic fracturing
fracking
Marcellus shale
16S rRNA gene sequencing
microbial communities
biomarkers
halophilic
hydrocarbons
2018-08-02 06:49:19
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_3_Bacterial_Biomarkers_of_Marcellus_Shale_Activity_in_Pennsylvania_XLSX/6892013
<p>Unconventional oil and gas (UOG) extraction, also known as hydraulic fracturing, is becoming more prevalent with the increasing use and demand for natural gas; however, the full extent of its environmental impacts is still unknown. Here we measured physicochemical properties and bacterial community composition of sediment samples taken from twenty-eight streams within the Marcellus shale formation in northeastern Pennsylvania differentially impacted by hydraulic fracturing activities. Fourteen of the streams were classified as UOG+, and thirteen were classified as UOG- based on the presence of UOG extraction in their respective watersheds. One stream was located in a watershed that previously had UOG extraction activities but was recently abandoned. We utilized high-throughput sequencing of the 16S rRNA gene to infer differences in sediment aquatic bacterial community structure between UOG+ and UOG- streams, as well as correlate bacterial community structure to physicochemical water parameters. Although overall alpha and beta diversity differences were not observed, there were a plethora of significantly enriched operational taxonomic units (OTUs) within UOG+ and UOG- samples. Our biomarker analysis revealed many of the bacterial taxa enriched in UOG+ streams can live in saline conditions, such as Rubrobacteraceae. In addition, several bacterial taxa capable of hydrocarbon degradation were also enriched in UOG+ samples, including Oceanospirillaceae. Methanotrophic taxa, such as Methylococcales, were significantly enriched as well. Several taxa that were identified as enriched in these samples were enriched in samples taken from different streams in 2014; moreover, partial least squares discriminant analysis (PLS-DA) revealed clustering between streams from the different studies based on the presence of hydraulic fracturing along the second axis. This study revealed significant differences between bacterial assemblages within stream sediments of UOG+ and UOG- streams and identified several potential biomarkers for evaluating and monitoring the response of autochthonous bacterial communities to potential hydraulic fracturing impacts.</p>