%0 Online Multimedia %A Pagano, Alessia %A Iaccarino, Nunzia %A Abdelhamid, Mahmoud A. S. %A Brancaccio, Diego %A Garzarella, Emanuele U. %A Di Porzio, Anna %A Novellino, Ettore %A Waller, Zoƫ A. E. %A Pagano, Bruno %A Amato, Jussara %A Randazzo, Antonio %D 2018 %T Presentation_1_Common G-Quadruplex Binding Agents Found to Interact With i-Motif-Forming DNA: Unexpected Multi-Target-Directed Compounds.PDF %U https://frontiersin.figshare.com/articles/presentation/Presentation_1_Common_G-Quadruplex_Binding_Agents_Found_to_Interact_With_i-Motif-Forming_DNA_Unexpected_Multi-Target-Directed_Compounds_PDF/6853934 %R 10.3389/fchem.2018.00281.s001 %2 https://frontiersin.figshare.com/ndownloader/files/12479939 %K G-quadruplex %K i-motif %K Berberine %K BRACO-19 %K Mitoxantrone %K Phen-DC3 %K Pyridostatin %K RHPS4 %X

G-quadruplex (G4) and i-motif (iM) are four-stranded non-canonical nucleic acid structural arrangements. Recent evidences suggest that these DNA structures exist in living cells and could be involved in several cancer-related processes, thus representing an attractive target for anticancer drug discovery. Efforts toward the development of G4 targeting compounds have led to a number of effective bioactive ligands. Herein, employing several biophysical methodologies, we studied the ability of some well-known G4 ligands to interact with iM-forming DNA. The data showed that the investigated compounds are actually able to interact with both DNA in vitro, thus acting de facto as multi-target-directed agents. Interestingly, while all the compounds stabilize the G4, some of them significantly reduce the stability of the iM. The present study highlights the importance, when studying G4-targeting compounds, of evaluating also their behavior toward the i-motif counterpart.

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