10.3389/fpls.2018.00913.s005 Zhengrong Zhang Zhengrong Zhang Xin Liu Xin Liu Rong Li Rong Li Li Yuan Li Yuan Yaqing Dai Yaqing Dai Xiaoyun Wang Xiaoyun Wang Image_5_Identification and Functional Analysis of a Protein Disulfide Isomerase (AtPDI1) in Arabidopsis thaliana.TIF Frontiers 2018 PDI isomerase activities stress resistance critical amino acids endoplasmic reticulum 2018-07-19 11:37:33 Figure https://frontiersin.figshare.com/articles/figure/Image_5_Identification_and_Functional_Analysis_of_a_Protein_Disulfide_Isomerase_AtPDI1_in_Arabidopsis_thaliana_TIF/6839879 <p>Protein disulfide isomerase (PDI) catalyzes the conversion of thiol-disulfide and plays an important role in various physiological events in animals. A PDI (OaPDI) from a tropical plant was detailed studied and it was found to be involved in response of biotic stress (Gruber et al., 2007). However, the activities of PDI related to physiological functions in plants are poorly understood. In the present study, a homolog of human PDI in Arabidopsis (AtPDI1), encoded by the gene (At3g54960), was characterized. The recombinant AtPDI1 protein had disulfide isomerase activity in vitro and two pairs of conservative cysteines in catalytic domains play a crucial role in the PDI activities. Expression of AtPDI1 in Escherichia coli significantly enhanced stress tolerance of cells and the mutations of critical cysteines almost lose this function. In plants, AtPDI1 was strongly induced by abiotic stresses and exogenous abscisic acid. An ArabidopsisAtPDI1 knockdown mutant (pdi1) and overexpression lines of transgenic plants obtained by this investigation were used to further examine the function of AtPDI1. The mutant line was more sensitive to stresses than the wild-type, while overexpressing AtPDI1 increased tolerance of seedlings to abiotic stresses, with a higher germination ratio and longer length of roots than the wild-type. Our results suggested AtPDI1 played roles in anti-stresses in Arabidopsis, which relate to the activities of PDI.</p>