10.3389/fpls.2018.01034.s005 Walter Chitarra Walter Chitarra Chiara Pagliarani Chiara Pagliarani Simona Abbà Simona Abbà Paolo Boccacci Paolo Boccacci Giancarlo Birello Giancarlo Birello Marika Rossi Marika Rossi Sabrina Palmano Sabrina Palmano Cristina Marzachì Cristina Marzachì Irene Perrone Irene Perrone Giorgio Gambino Giorgio Gambino Table_3_miRVIT: A Novel miRNA Database and Its Application to Uncover Vitis Responses to Flavescence dorée Infection.XLSX Frontiers 2018 disease resistance jasmonate miRNAs phytoplasma target genes univocal database 2018-07-17 14:01:55 Dataset https://frontiersin.figshare.com/articles/dataset/Table_3_miRVIT_A_Novel_miRNA_Database_and_Its_Application_to_Uncover_Vitis_Responses_to_Flavescence_dor_e_Infection_XLSX/6826109 <p>Micro(mi)RNAs play crucial roles in plant developmental processes and in defense responses to biotic and abiotic stresses. In the last years, many works on small RNAs in grapevine (Vitis spp.) were published, and several conserved and putative novel grapevine-specific miRNAs were identified. In order to reorganize the high quantity of available data, we produced “miRVIT,” the first database of all novel grapevine miRNA candidates characterized so far, and still not deposited in miRBase. To this aim, each miRNA accession was renamed, repositioned in the last version of the grapevine genome, and compared with all the novel and conserved miRNAs detected in grapevine. Conserved and novel miRNAs cataloged in miRVIT were then used for analyzing Vitis vinifera plants infected by Flavescence dorée (FD), one of the most severe phytoplasma diseases affecting grapevine. The analysis of small RNAs from healthy, recovered (plants showing spontaneous and stable remission of symptoms), and FD-infected “Barbera” grapevines showed that FD altered the expression profiles of several miRNAs, including those involved in cell development and photosynthesis, jasmonate signaling, and disease resistance response. The application of miRVIT in a biological context confirmed the effectiveness of the followed approach, especially for the identification of novel miRNA candidates in grapevine. miRVIT database is available at http://mirvit.ipsp.cnr.it.</p><p>Highlights: The application of the newly produced database of grapevine novel miRNAs to the analysis of plants infected by Flavescence dorée reveals key roles of miRNAs in photosynthesis and jasmonate signaling.</p>