10.3389/fpls.2018.01018.s011 Binghao Bao Binghao Bao Hongbo Chao Hongbo Chao Hao Wang Hao Wang Weiguo Zhao Weiguo Zhao Lina Zhang Lina Zhang Nadia Raboanatahiry Nadia Raboanatahiry Xiaodong Wang Xiaodong Wang Baoshan Wang Baoshan Wang Haibo Jia Haibo Jia Maoteng Li Maoteng Li Table_9_Stable, Environmental Specific and Novel QTL Identification as Well as Genetic Dissection of Fatty Acid Metabolism in Brassica napus.XLSX Frontiers 2018 Brassica napus fatty acid QTL mapping acyl lipid metabolism genetic dissection 2018-07-17 13:58:29 Dataset https://frontiersin.figshare.com/articles/dataset/Table_9_Stable_Environmental_Specific_and_Novel_QTL_Identification_as_Well_as_Genetic_Dissection_of_Fatty_Acid_Metabolism_in_Brassica_napus_XLSX/6826091 <p>Fatty acid (FA) composition is the typical quantitative trait in oil seed crops, of which study is not only closely related to oil content, but is also more critical for the quality improvement of seed oil. The double haploid (DH) population named KN with a high density SNP linkage map was applied for quantitative trait loci (QTL) analysis of FA composition in this study. A total of 406 identified QTL were detected for eight FA components with an average confidence interval (CI) of 2.92 cM, the explained phenotypic variation (PV) value ranged from 1.49 to 45.05%. Totally, 204 consensus and 91 unique QTL were further obtained via meta-analysis method for the purpose of detecting multiple environment expressed and pleiotropic QTL, respectively. Of which, 74 stable expressed and 22 environmental specific QTL were also revealed, respectively. In order to make clear the genetic mechanism of FA metabolism at individual QTL level, conditional QTL analysis was also conducted and more than two thousand conditional QTL which could not be detected under the unconditional mapping were detected, which indicated the complex interrelationship of the QTL controlling FA content in rapeseed. Through comparative genomic analysis and homologous gene annotation, 61 candidates related to acyl lipid metabolism were identified underlying the CI of FA QTL. To further visualize the genetic mechanism of FA metabolism, an intuitive and meticulous network about acyl lipid metabolism was constructed and some closely related candidates were positioned. This study provided a more accurate localization for stable and pleiotropic QTL, and a deeper dissection of the molecular regulatory mechanism of FA metabolism in rapeseed.</p>