10.3389/fpls.2018.01018.s001
Binghao Bao
Binghao
Bao
Hongbo Chao
Hongbo
Chao
Hao Wang
Hao
Wang
Weiguo Zhao
Weiguo
Zhao
Lina Zhang
Lina
Zhang
Nadia Raboanatahiry
Nadia
Raboanatahiry
Xiaodong Wang
Xiaodong
Wang
Baoshan Wang
Baoshan
Wang
Haibo Jia
Haibo
Jia
Maoteng Li
Maoteng
Li
Image_1_Stable, Environmental Specific and Novel QTL Identification as Well as Genetic Dissection of Fatty Acid Metabolism in Brassica napus.JPEG
Frontiers
2018
Brassica napus
fatty acid
QTL mapping
acyl lipid metabolism
genetic dissection
2018-07-17 13:58:26
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Stable_Environmental_Specific_and_Novel_QTL_Identification_as_Well_as_Genetic_Dissection_of_Fatty_Acid_Metabolism_in_Brassica_napus_JPEG/6826058
<p>Fatty acid (FA) composition is the typical quantitative trait in oil seed crops, of which study is not only closely related to oil content, but is also more critical for the quality improvement of seed oil. The double haploid (DH) population named KN with a high density SNP linkage map was applied for quantitative trait loci (QTL) analysis of FA composition in this study. A total of 406 identified QTL were detected for eight FA components with an average confidence interval (CI) of 2.92 cM, the explained phenotypic variation (PV) value ranged from 1.49 to 45.05%. Totally, 204 consensus and 91 unique QTL were further obtained via meta-analysis method for the purpose of detecting multiple environment expressed and pleiotropic QTL, respectively. Of which, 74 stable expressed and 22 environmental specific QTL were also revealed, respectively. In order to make clear the genetic mechanism of FA metabolism at individual QTL level, conditional QTL analysis was also conducted and more than two thousand conditional QTL which could not be detected under the unconditional mapping were detected, which indicated the complex interrelationship of the QTL controlling FA content in rapeseed. Through comparative genomic analysis and homologous gene annotation, 61 candidates related to acyl lipid metabolism were identified underlying the CI of FA QTL. To further visualize the genetic mechanism of FA metabolism, an intuitive and meticulous network about acyl lipid metabolism was constructed and some closely related candidates were positioned. This study provided a more accurate localization for stable and pleiotropic QTL, and a deeper dissection of the molecular regulatory mechanism of FA metabolism in rapeseed.</p>