10.3389/fpls.2018.00885.s002
Jianzhong Wu
Jianzhong
Wu
Qian Zhao
Qian
Zhao
Liyan Zhang
Liyan
Zhang
Suiyan Li
Suiyan
Li
Yanhua Ma
Yanhua
Ma
Liyan Pan
Liyan
Pan
Hong Lin
Hong
Lin
Guangwen Wu
Guangwen
Wu
Hongmei Yuan
Hongmei
Yuan
Ying Yu
Ying
Yu
Xun Wang
Xun
Wang
Xue Yang
Xue
Yang
Zhugang Li
Zhugang
Li
Tingbo Jiang
Tingbo
Jiang
Dequan Sun
Dequan
Sun
Presentation_2_QTL Mapping of Fiber-Related Traits Based on a High-Density Genetic Map in Flax (Linum usitatissimum L.).ZIP
Frontiers
2018
Linum usitatissimum L.
specific-locus amplified fragment (SLAF)
high-density genetic map
quantitative trait loci (QTL)
flax
2018-07-17 04:38:02
Presentation
https://frontiersin.figshare.com/articles/presentation/Presentation_2_QTL_Mapping_of_Fiber-Related_Traits_Based_on_a_High-Density_Genetic_Map_in_Flax_Linum_usitatissimum_L_ZIP/6824552
<p>A genetic map is an important and valuable tool for quantitative trait locus (QTL) mapping, marker-assisted selection (MAS)-based breeding, and reference-assisted chromosome assembly. In this study, 112 F<sub>2</sub> plants from a cross between Linum usitatissimum L. “DIANE” and “NY17” and parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 61.64 Gb of raw data containing 253.71 Mb paired-end reads, each 101 bp in length, were obtained. A total of 192,797 SLAFs were identified, of which 23,115 were polymorphic, with a polymorphism rate of 11.99%. Finally, 2,339 SLAFs were organized into a linkage map consisting of 15 linkage groups (LGs). The total length of the genetic map was 1483.25 centimorgans (cM) and the average distance between adjacent markers was 0.63 cM. Combined with flax chromosome-scale pseudomolecules, 12 QTLs associating with 6 flax fiber-related traits were mapped on the chromosomal scaffolds. This high-density genetic map of flax should serve as a foundation for flax fine QTL mapping, draft genome assembly, and MAS-guided breeding. Ultimately, the genomic regions identified in this research could potentially be valuable for improving flax fiber cultivars, as well as for identification of candidate genes involved in flax fiber formation processes.</p>Significance statement<p>A high-density genetic map of flax was constructed, and QTLs were identified on the sequence scaffolds to be interrelated with fiber-related traits. The results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for flax, but also provide a reference to help position sequence scaffolds on the physical map and assist in the process of assembling the flax genome sequence.</p>