10.3389/fnmol.2018.00234.s001 Gerry Nganou Gerry Nganou Carla G. Silva Carla G. Silva Ivan Gladwyn-Ng Ivan Gladwyn-Ng Dominique Engel Dominique Engel Bernard Coumans Bernard Coumans Antonio V. Delgado-Escueta Antonio V. Delgado-Escueta Miyabi Tanaka Miyabi Tanaka Laurent Nguyen Laurent Nguyen Thierry Grisar Thierry Grisar Laurence de Nijs Laurence de Nijs Bernard Lakaye Bernard Lakaye Data_Sheet_1_Importin-8 Modulates Division of Apical Progenitors, Dendritogenesis and Tangential Migration During Development of Mouse Cortex.PDF Frontiers 2018 karyopherin importin-8 corticogenesis radial migration tangential migration dendritogenesis in utero electroporation 2018-07-10 04:16:45 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Importin-8_Modulates_Division_of_Apical_Progenitors_Dendritogenesis_and_Tangential_Migration_During_Development_of_Mouse_Cortex_PDF/6794738 <p>The building of the brain is a multistep process that requires the coordinate expression of thousands of genes and an intense nucleocytoplasmic transport of RNA and proteins. This transport is mediated by karyopherins that comprise importins and exportins. Here, we investigated the role of the ß-importin, importin-8 (IPO8) during mouse cerebral corticogenesis as several of its cargoes have been shown to be essential during this process. First, we showed that Ipo8 mRNA is expressed in mouse brain at various embryonic ages with a clear signal in the sub-ventricular/ventricular zone (SVZ/VZ), the cerebral cortical plate (CP) and the ganglionic eminences. We found that acute knockdown of IPO8 in cortical progenitors reduced both their proliferation and cell cycle exit leading to the increase in apical progenitor pool without influencing the number of basal progenitors (BPs). Projection neurons ultimately reached their appropriate cerebral cortical layer, but their dendritogenesis was specifically affected, resulting in neurons with reduced dendrite complexity. IPO8 knockdown also slowed the migration of cortical interneurons. Together, our data demonstrate that IPO8 contribute to the coordination of several critical steps of cerebral cortex development. These results suggest that the impairment of IPO8 function might be associated with some diseases of neuronal migration defects.</p>