10.3389/fnmol.2018.00234.s001
Gerry Nganou
Gerry
Nganou
Carla G. Silva
Carla
G. Silva
Ivan Gladwyn-Ng
Ivan
Gladwyn-Ng
Dominique Engel
Dominique
Engel
Bernard Coumans
Bernard
Coumans
Antonio V. Delgado-Escueta
Antonio V.
Delgado-Escueta
Miyabi Tanaka
Miyabi
Tanaka
Laurent Nguyen
Laurent
Nguyen
Thierry Grisar
Thierry
Grisar
Laurence de Nijs
Laurence
de Nijs
Bernard Lakaye
Bernard
Lakaye
Data_Sheet_1_Importin-8 Modulates Division of Apical Progenitors, Dendritogenesis and Tangential Migration During Development of Mouse Cortex.PDF
Frontiers
2018
karyopherin
importin-8
corticogenesis
radial migration
tangential migration
dendritogenesis
in utero electroporation
2018-07-10 04:16:45
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Importin-8_Modulates_Division_of_Apical_Progenitors_Dendritogenesis_and_Tangential_Migration_During_Development_of_Mouse_Cortex_PDF/6794738
<p>The building of the brain is a multistep process that requires the coordinate expression of thousands of genes and an intense nucleocytoplasmic transport of RNA and proteins. This transport is mediated by karyopherins that comprise importins and exportins. Here, we investigated the role of the ß-importin, importin-8 (IPO8) during mouse cerebral corticogenesis as several of its cargoes have been shown to be essential during this process. First, we showed that Ipo8 mRNA is expressed in mouse brain at various embryonic ages with a clear signal in the sub-ventricular/ventricular zone (SVZ/VZ), the cerebral cortical plate (CP) and the ganglionic eminences. We found that acute knockdown of IPO8 in cortical progenitors reduced both their proliferation and cell cycle exit leading to the increase in apical progenitor pool without influencing the number of basal progenitors (BPs). Projection neurons ultimately reached their appropriate cerebral cortical layer, but their dendritogenesis was specifically affected, resulting in neurons with reduced dendrite complexity. IPO8 knockdown also slowed the migration of cortical interneurons. Together, our data demonstrate that IPO8 contribute to the coordination of several critical steps of cerebral cortex development. These results suggest that the impairment of IPO8 function might be associated with some diseases of neuronal migration defects.</p>