10.3389/fphys.2018.00804.s001
Martin Golan
Martin
Golan
Sarka Jelinkova
Sarka
Jelinkova
Irena Kratochvílová
Irena
Kratochvílová
Petr Skládal
Petr
Skládal
Martin Pešl
Martin
Pešl
Vladimír Rotrekl
Vladimír
Rotrekl
Jan Pribyl
Jan
Pribyl
Data_Sheet_1_AFM Monitoring the Influence of Selected Cryoprotectants on Regeneration of Cryopreserved Cells Mechanical Properties.docx
Frontiers
2018
cryopreservation
cell stiffness
AFM
fluorescence microscopy
DMSO
PEG
2018-06-29 10:48:07
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_AFM_Monitoring_the_Influence_of_Selected_Cryoprotectants_on_Regeneration_of_Cryopreserved_Cells_Mechanical_Properties_docx/6724412
<p>Cryopreservation of cells (mouse embryonic fibroblasts) is a fundamental task for wide range of applications. In practice, cells are protected against damage during freezing by applications of specific cryoprotectants and freezing/melting protocols. In this study by using AFM and fluorescence microscopy we showed how selected cryoprotectants (dimethyl sulfoxide and polyethylene glycol) affected the cryopreserved cells mechanical properties (stiffness) and how these parameters are correlated with cytoskeleton damage and reconstruction. We showed how cryopreserved (frozen and thawed) cells' stiffness change according to type of applied cryoprotectant and its functionality in extracellular or intracellular space. We showed that AFM can be used as technique for investigation of cryopreserved cells surfaces state and development ex vivo. Our results offer a new perspective on the monitoring and characterization of frozen cells recovery by measuring changes in elastic properties by nanoindentation technique. This may lead to a new and detailed way of investigating the post-thaw development of cryopreserved cells which allows to distinguish between different cell parts.</p>