Data_Sheet_2_Bid Expression Network Controls Neuronal Cell Fate During Avian Ciliary Ganglion Development.ZIP Sophie Koszinowski Veronica La Padula Frank Edlich Kerstin Krieglstein Hauke Busch Melanie Boerries 10.3389/fphys.2018.00797.s002 https://frontiersin.figshare.com/articles/dataset/Data_Sheet_2_Bid_Expression_Network_Controls_Neuronal_Cell_Fate_During_Avian_Ciliary_Ganglion_Development_ZIP/6723644 <p>Avian ciliary ganglion (CG) development involves a transient execution phase of apoptosis controlling the final number of neurons, but the time-dependent molecular mechanisms for neuronal cell fate are largely unknown. To elucidate the molecular networks regulating important aspects of parasympathetic neuronal development, a genome-wide expression analysis was performed during multiple stages of avian CG development between embryonic days E6 and E14. The transcriptome data showed a well-defined sequence of events, starting from neuronal migration via neuronal fate cell determination, synaptic transmission, and regulation of synaptic plasticity to growth factor associated signaling. In particular, we extracted a neuronal apoptosis network that characterized the cell death execution phase at E8/E9 and apoptotic cell clearance at E14 by combining the gene time series analysis with network synthesis from the chicken interactome. Network analysis identified TP53 as key regulator and predicted involvement of the BH3 interacting domain death agonist (BID). A virus-based RNAi knockdown approach in vivo showed a crucial impact of BID expression on the execution of ontogenetic programmed cell death (PCD). In contrast, Bcl-XL expression did not impact PCD. Therefore, BID-mediated apoptosis represents a novel cue essential for timing within CG maturation.</p> 2018-06-29 08:37:17 network inference protein interaction network ontogenetic cell death neuronal development BID time series analysis