10.3389/fpls.2018.00863.s001
Meng Zhang
Meng
Zhang
Xiaofei Jin
Xiaofei
Jin
Ying Chen
Ying
Chen
Mi Wei
Mi
Wei
Weifang Liao
Weifang
Liao
Shengying Zhao
Shengying
Zhao
Chunhua Fu
Chunhua
Fu
Longjiang Yu
Longjiang
Yu
Table_1_TcMYC2a, a Basic Helix–Loop–Helix Transcription Factor, Transduces JA-Signals and Regulates Taxol Biosynthesis in Taxus chinensis.XLSX
Frontiers
2018
TcMYC2a
TcJAZ3
JA signaling pathway
tasy gene
Taxus chinensis
2018-06-21 04:24:50
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_TcMYC2a_a_Basic_Helix_Loop_Helix_Transcription_Factor_Transduces_JA-Signals_and_Regulates_Taxol_Biosynthesis_in_Taxus_chinensis_XLSX/6628094
<p>The multitherapeutic taxol, which can be obtained from Taxus spp., is the most widely used anticancer drug. Taxol biosynthesis is significantly regulated by jasmonate acid (JA), one of the most important endogenous hormones in land plants. Nevertheless, the JA-inducing mechanism remains poorly understood. MYC2 is one of the key regulators of JA signal transfer and the biosynthesis of various secondary metabolites. Here, TcMYC2a was identified to contain a basic helix–loop–helix (bHLH)-leucine zipper domain, a bHLH-MYC_N domain, and a BIF/ACT-like domain. TcMYC2a was also found to bind with TcJAZ3 in yeast, which was a homolog of Arabidopsis JASMONATE ZIM-domain JAZ proteins, indicating that TcMYC2a had a similar function to AtMYC2 of JA signal transduction. TcMYC2a was able to affect the expression of GUS reporter gene by binding with the T/G-box, G-box, and E-box, which were the key cis-elements of TASY and TcERF12/15 promoter. TcMYC2a overexpression also led to significantly increased expression of TASY, tat, dbtnbt, t13h, and t5h genes. Additionally, TcERF15, which played the positive role to regulate tasy gene, was up-regulated by TcMYC2a. All these results revealed that TcMYC2a can regulate taxol biosynthesis either directly or via ERF regulators depending on JA signaling transduction.</p>