10.3389/fcimb.2018.00202.s001 Juan-Juan Gao Juan-Juan Gao Yang Zhang Yang Zhang Markus Gerhard Markus Gerhard Raquel Mejias-Luque Raquel Mejias-Luque Lian Zhang Lian Zhang Michael Vieth Michael Vieth Jun-Ling Ma Jun-Ling Ma Monther Bajbouj Monther Bajbouj Stepan Suchanek Stepan Suchanek Wei-Dong Liu Wei-Dong Liu Kurt Ulm Kurt Ulm Michael Quante Michael Quante Zhe-Xuan Li Zhe-Xuan Li Tong Zhou Tong Zhou Roland Schmid Roland Schmid Meinhard Classen Meinhard Classen Wen-Qing Li Wen-Qing Li Wei-Cheng You Wei-Cheng You Kai-Feng Pan Kai-Feng Pan Data_Sheet_1_Association Between Gut Microbiota and Helicobacter pylori-Related Gastric Lesions in a High-Risk Population of Gastric Cancer.docx Frontiers 2018 Helicobacter pylori gastric lesions gut microbiota microbial diversity 16S ribosomal RNA gene sequencing 2018-06-19 04:38:39 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Association_Between_Gut_Microbiota_and_Helicobacter_pylori-Related_Gastric_Lesions_in_a_High-Risk_Population_of_Gastric_Cancer_docx/6599087 <p>Eradication of Helicobacter pylori has been found to be effective for gastric cancer prevention, but uncertainties remain about the possible adverse consequences such as the potential microbial dysbiosis. In our study, we investigated the association between gut microbiota and H. pylori-related gastric lesions in 47 subjects by deep sequencing of microbial 16S ribosomal RNA (rRNA) gene in fecal samples. The dominant phyla in fecal samples were Bacteroidetes, Firmicutes, and Proteobacteria with average relative abundances of 54.77, 31.37 and 12.91%, respectively. Microbial diversity analysis showed that observed species and Shannon index were increased in subjects with past or current H. pylori infection compared with negative subjects. As for the differential bacteria, the average relative abundance of Bacteroidetes was found to significantly decrease from H. pylori negative (66.16%) to past infection group (33.01%, p = 0.007), as well as from normal (76.49%) to gastritis (56.04%) and metaplasia subjects (46.83%, p = 0.027). For Firmicutes and Proteobacteria, the average relative abundances showed elevated trends in the past H. pylori infection group (47.11, 20.53%) compared to negative group (23.44, 9.05%, p = 0.068 and 0.246, respectively), and similar increased trends were also found from normal (18.23, 5.05%) to gastritis (35.31, 7.23%, p = 0.016 and 0.294, respectively) or metaplasia subjects (32.33, 20.07%, both p < 0.05). These findings suggest that the alterations of fecal microbiota, especially the dominant phyla of Bacteroidetes, Firmicutes and Proteobacteria, may be involved in the process of H. pylori-related gastric lesion progression and provide hints for future evaluation of microbial changes after H. pylori eradication.</p>