10.3389/fnmol.2018.00211.s001
Elli-Anna Balta
Elli-Anna
Balta
Marie-Theres Wittmann
Marie-Theres
Wittmann
Matthias Jung
Matthias
Jung
Elisabeth Sock
Elisabeth
Sock
Benjamin Martin Haeberle
Benjamin Martin
Haeberle
Birgit Heim
Birgit
Heim
Felix von Zweydorf
Felix von
Zweydorf
Jana Heppt
Jana
Heppt
Julia von Wittgenstein
Julia
von Wittgenstein
Christian Johannes Gloeckner
Christian Johannes
Gloeckner
Dieter Chichung Lie
Dieter Chichung
Lie
Image_1_Phosphorylation Modulates the Subcellular Localization of SOX11.TIF
Frontiers
2018
SOX11
transcription factor phosphorylation
subcellular localization
neurogenesis
cancer
intellectual disability
2018-06-19 04:05:20
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Phosphorylation_Modulates_the_Subcellular_Localization_of_SOX11_TIF/6597920
<p>SOX11 is a key Transcription Factor (TF) in the regulation of embryonic and adult neurogenesis, whose mutation has recently been linked to an intellectual disability syndrome in humans. SOX11’s transient activity during neurogenesis is critical to ensure the precise execution of the neurogenic program. Here, we report that SOX11 displays differential subcellular localizations during the course of neurogenesis. Western-Blot analysis of embryonic mouse brain lysates indicated that SOX11 is post-translationally modified by phosphorylation. Using Mass Spectrometry, we found 10 serine residues in the SOX11 protein that are putatively phosphorylated. Systematic analysis of phospho-mutant SOX11 resulted in the identification of the S30 residue, whose phosphorylation promotes nuclear over cytoplasmic localization of SOX11. Collectively, these findings uncover phosphorylation as a novel layer of regulation of the intellectual disability gene Sox11.</p>