Image_1_Antibacterial Activity and Mechanism of Action of Aspidinol Against Multi-Drug-Resistant Methicillin-Resistant Staphylococcus aureus.TIF Xin Hua Qin Yang Wanjiang Zhang Zhimin Dong Shenye Yu Stefan Schwarz Siguo Liu 10.3389/fphar.2018.00619.s001 https://frontiersin.figshare.com/articles/figure/Image_1_Antibacterial_Activity_and_Mechanism_of_Action_of_Aspidinol_Against_Multi-Drug-Resistant_Methicillin-Resistant_Staphylococcus_aureus_TIF/6505271 <p>This study aimed at investigating the antibacterial activity of aspidinol, an extract from Dryopteris fragrans (L.) Schott, against methicillin-resistant Staphylococcus aureus (MRSA). MRSA isolates were treated with aspidinol to determine the differential expression of genes and associated pathways following the drug treatment. Aspidinol displayed significant anti-MRSA activity, both in vivo (minimum inhibitory concentration = 2 μg/mL) and in vitro, and achieved an antibacterial effect comparable to that of vancomycin. In the lethal septicemic mouse study, a dose of 50 mg/kg of either aspidinol or vancomycin provided significant protection from mortality. In the non-lethal septicemic mouse study, aspidinol and vancomycin produced a significant reduction in mean bacterial load in murine organs, including the spleen, lung, and liver. After treatment with aspidinol, we found through RNA-seq and RT-PCR experiments that the inhibition of the formation of ribosomes was the primary S. aureus cell-killing mechanism, and the inhibition of amino acid synthesis and the reduction of virulence factors might play a secondary role.</p> 2018-06-13 04:13:12 aspidinol anti-MRSA activity antimicrobial mechanism RNA-seq inhibit ribosomes synthesis