10.3389/fcimb.2018.00195.s001
Yang He
Yang
He
Mingshu Wang
Mingshu
Wang
Mafeng Liu
Mafeng
Liu
Li Huang
Li
Huang
Chaoyue Liu
Chaoyue
Liu
Xin Zhang
Xin
Zhang
Haibo Yi
Haibo
Yi
Anchun Cheng
Anchun
Cheng
Dekang Zhu
Dekang
Zhu
Qiao Yang
Qiao
Yang
Ying Wu
Ying
Wu
Xinxin Zhao
Xinxin
Zhao
Shun Chen
Shun
Chen
Renyong Jia
Renyong
Jia
Shaqiu Zhang
Shaqiu
Zhang
Yunya Liu
Yunya
Liu
Yanling Yu
Yanling
Yu
Ling Zhang
Ling
Zhang
Image_1_Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition.PDF
Frontiers
2018
Riemerella anatipestifer
CRISPR-Cas
Cas1
Cas2
spacer acquisition
2018-06-12 12:33:07
Figure
https://frontiersin.figshare.com/articles/figure/Image_1_Cas1_and_Cas2_From_the_Type_II-C_CRISPR-Cas_System_of_Riemerella_anatipestifer_Are_Required_for_Spacer_Acquisition_PDF/6491354
<p>Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide acquired genetic immunity against the entry of mobile genetic elements (MGEs). The immune defense provided by various subtypes of the CRISPR-Cas system has been confirmed and is closely associated with the formation of immunological memory in CRISPR arrays, called CRISPR adaptation or spacer acquisition. However, whether type II-C CRISPR-Cas systems are also involved in spacer acquisition remains largely unknown. This study explores and provides some definitive evidence regarding spacer acquisition of the type II-C CRISPR-Cas system from Riemerella anatipestifer (RA) CH-2 (RA-CH-2). Firstly, introducing an exogenous plasmid into RA-CH-2 triggered spacer acquisition of RA CRISPR-Cas system, and the acquisition of new spacers led to plasmid instability in RA-CH-2. Furthermore, deletion of cas1 or cas2 of RA-CH-2 abrogated spacer acquisition and subsequently stabilized the exogenous plasmid, suggesting that both Cas1 and Cas2 are required for spacer acquisition of RA-CH-2 CRISPR-Cas system, consistent with the reported role of Cas1 and Cas2 in type I-E and II-A systems. Finally, assays for studying Cas1 nuclease activity and the interaction of Cas1 with Cas2 contributed to a better understanding of the adaptation mechanism of RA CRISPR-Cas system. This is the first experimental identification of the naïve adaptation of type II-C CRISPR-Cas system.</p>