10.3389/fneur.2018.00407.s004
Tillmann Ruland
Tillmann
Ruland
Jolien Wolbert
Jolien
Wolbert
Michael G. Gottschalk
Michael G.
Gottschalk
Simone König
Simone
König
Andreas Schulte-Mecklenbeck
Andreas
Schulte-Mecklenbeck
Jens Minnerup
Jens
Minnerup
Sven G. Meuth
Sven
G. Meuth
Catharina C. Groß
Catharina C.
Groß
Heinz Wiendl
Heinz
Wiendl
Gerd Meyer zu Hörste
Gerd
Meyer zu Hörste
Table_4_Cerebrospinal Fluid Concentrations of Neuronal Proteins Are Reduced in Primary Angiitis of the Central Nervous System.DOCX
Frontiers
2018
PACNS
CSF
mass spectrometry
flow cytometry
amyloid-beta A4 protein
2018-06-05 04:36:14
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_4_Cerebrospinal_Fluid_Concentrations_of_Neuronal_Proteins_Are_Reduced_in_Primary_Angiitis_of_the_Central_Nervous_System_DOCX/6443345
<p>Primary angiitis of the central nervous system (PACNS) is a rare autoimmune vasculitis limited to the CNS often causing substantial disability. Understanding of this disease is impaired by the lack of available biomaterial. Here, we collected cerebrospinal fluid (CSF) from patients with PACNS and matched controls and performed unbiased proteomics profiling using ion mobility mass spectrometry to identify novel disease mechanisms and candidate biomarkers. We identified 14 candidate proteins, including amyloid-beta A4 protein (APP), with reduced abundance in the CSF of PACNS patients and validated APP by Enzyme-linked Immunosorbent Assay (ELISA) in an extended cohort of patients with PACNS. Subsequent functional annotation surprisingly suggested neuronal pathology rather than immune activation in PACNS. Our study is the first to employ mass spectrometry to local immune reactions in PACNS and it identifies candidates such as APP with pathogenic relevance in PACNS to improve patient care in the future.</p>