%0 Generic %A Lai, Li-Yin %A Lin, Tzu-Lung %A Chen, Yi-Yin %A Hsieh, Pei-Fang %A Wang, Jin-Town %D 2018 %T Data_Sheet_1_Role of the Mycobacterium marinum ESX-1 Secretion System in Sliding Motility and Biofilm Formation.pdf %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Role_of_the_Mycobacterium_marinum_ESX-1_Secretion_System_in_Sliding_Motility_and_Biofilm_Formation_pdf/6388979 %R 10.3389/fmicb.2018.01160.s001 %2 https://frontiersin.figshare.com/ndownloader/files/11762444 %K type VII secretion system %K Mycobacterium marinum %K ESX-1 %K sliding motility %K biofilm formation %X

Mycobacterium marinum is a close relative of Mycobacterium tuberculosis that can cause systemic tuberculosis-like infections in ectotherms and skin infections in humans. Sliding motility correlates with biofilm formation and virulence in most bacteria. In this study, we used a sliding motility assay to screen 2,304 transposon mutants of M. marinum NTUH-M6885 and identified five transposon mutants with decreased sliding motility. Transposons that interrupted the type VII secretion system (T7SS) ESX-1-related genes, espE (mmar_5439), espF (mmar_5440), and eccA1 (mmar_5443), were present in 3 mutants. We performed reverse-transcription polymerase chain reaction to verify genes from mmar_5438 to mmar_5450, which were found to belong to a single transcriptional unit. Deletion mutants of espE, espF, espG (mmar_5441), and espH (mmar_5442) displayed significant attenuation regarding sliding motility and biofilm formation. M. marinum NTUH-M6885 possesses a functional ESX-1 secretion system. However, deletion of espG or espH resulted in slightly decreased secretion of EsxB (which is also known as CFP-10). Thus, the M. marinum ESX-1 secretion system mediates sliding motility and is crucial for biofilm formation. These data provide new insight into M. marinum biofilm formation.

%I Frontiers