%0 Generic %A Prerostova, Sylva %A I. Dobrev, Petre %A Gaudinova, Alena %A Knirsch, Vojtech %A Körber, Niklas %A Pieruschka, Roland %A Fiorani, Fabio %A Brzobohatý, Břetislav %A černý, Martin %A Spichal, Lukas %A Humplik, Jan %A Vanek, Tomas %A Schurr, Ulrich %A Vankova, Radomira %D 2018 %T Data_Sheet_1_Cytokinins: Their Impact on Molecular and Growth Responses to Drought Stress and Recovery in Arabidopsis.pdf %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Cytokinins_Their_Impact_on_Molecular_and_Growth_Responses_to_Drought_Stress_and_Recovery_in_Arabidopsis_pdf/6303866 %R 10.3389/fpls.2018.00655.s001 %2 https://frontiersin.figshare.com/ndownloader/files/11542031 %K abscisic acid %K auxin %K cytokinin %K cytokinin oxidase/dehydrogenase %K drought stress %K isopentenyl transferase %K phytohormone %X

Our phenotyping and hormonal study has characterized the role of cytokinins (CK) in the drought and recovery responses of Arabidopsis thaliana. CK down-regulation was achieved by overexpression of the gene for CK deactivating enzyme cytokinin oxidase/dehydrogenase (CKX): constitutive (35S:CKX) or at the stress onset using a dexamethasone-inducible pOp/LhGR promoter (DEX:CKX). The 35S:CKX plants exhibited slow ontogenesis and higher expression levels of stress-associated genes, e.g., AtP5CS1, already at well-watered conditions. CK down-regulation resulted during drought in higher stress tolerance (indicated by relatively low up-regulation of the expression of drought stress marker gene AtRD29B) accompanied with lower leaf water loss. Nevertheless, these plants exhibited slow and delayed recovery after re-watering. CK levels were increased at the stress onset by stimulation of the expression of CK biosynthetic gene isopentenyl transferase (ipt) (DEX:IPT) or by application of exogenous CK meta-topolin. After water withdrawal, long-term CK elevation resulted in higher water loss in comparison with CKX transformants as well as with plants overexpressing ipt driven by senescence-inducible SAG12 promoter (SAG:IPT), which gradually enhanced CKs during the stress progression. In all cases, CK up-regulation resulted in fast and more vigorous recovery. All drought-stressed plants exhibited growth suppression associated with elevation of abscisic acid and decrease of auxins and active CKs (with the exception of SAG:IPT plants). Apart from the ipt overexpressers, also increase of jasmonic and salicylic acid was found.

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