%0 Generic %A Piekarski, Alissa %A Nagarajan, Gurueswar %A Ishola, Peter %A Flees, Joshua %A Greene, Elizabeth S. %A J. Kuenzel, Wayne %A Ohkubo, Takeshi %A Maier, Helena %A Bottje, Walter G. %A Cline, Mark A. %A Dridi, Sami %D 2018 %T Data_Sheet_1_AMP-Activated Protein Kinase Mediates the Effect of Leptin on Avian Autophagy in a Tissue-Specific Manner.DOCX %U https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_AMP-Activated_Protein_Kinase_Mediates_the_Effect_of_Leptin_on_Avian_Autophagy_in_a_Tissue-Specific_Manner_DOCX/6269630 %R 10.3389/fphys.2018.00541.s001 %2 https://frontiersin.figshare.com/ndownloader/files/11456378 %K leptin %K autophagy %K AMPK %K ICV injection %K cell cultures %K chickens %X

Autophagy, a highly conserved intracellular self-digestion process, plays an integral role in maintaining cellular homeostasis. Although emerging evidence indicate that the endocrine system regulates autophagy in mammals, there is still a scarcity of information on autophagy in avian (non-mammalian) species. Here, we show that intracerebroventricular administration of leptin reduces feed intake, modulates the expression of feeding-related hypothalamic neuropeptides, activates leptin receptor and signal transducer and activator of transcription (Ob-Rb/STAT) pathway, and significantly increases the expression of autophagy-related proteins (Atg3, Atg5, Atg7, beclin1, and LC3B) in chicken hypothalamus, liver, and muscle. Similarly, leptin treatment activates Ob-Rb/STAT pathway and increased the expression of autophagy-related markers in chicken hypothalamic organotypic cultures, muscle (QM7) and hepatocyte (Sim-CEL) cell cultures as well as in Chinese Hamster Ovary (CHO-K1) cells-overexpressing chicken Ob-Rb and STAT3. To define the downstream mediator(s) of leptin's effects on autophagy, we determined the role of the master energy sensor AMP-activated protein kinase (AMPK). Leptin treatment significantly increased the phosphorylated levels of AMPKα1/2 at Thr172 site in chicken hypothalamus and liver, but not in muscle. Likewise, AMPKα1/2 was activated by leptin in chicken hypothalamic organotypic culture and Sim-CEL, but not in QM7 cells. Blocking AMPK activity by compound C reverses the autophagy-inducing effect of leptin. Together, these findings indicate that AMPK mediates the effect of leptin on chicken autophagy in a tissue-specific manner.

%I Frontiers