10.3389/fmicb.2018.00988.s001
Ping Jiang
Ping
Jiang
Shujun Zheng
Shujun
Zheng
Ling Lu
Ling
Lu
Data_Sheet_1_Mitotic-Spindle Organizing Protein MztA Mediates Septation Signaling by Suppressing the Regulatory Subunit of Protein Phosphatase 2A-ParA in Aspergillus nidulans.PDF
Frontiers
2018
ParA
MztA
PP2A
suppressor
septation
Aspergillus nidulans
2018-05-08 04:29:20
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Mitotic-Spindle_Organizing_Protein_MztA_Mediates_Septation_Signaling_by_Suppressing_the_Regulatory_Subunit_of_Protein_Phosphatase_2A-ParA_in_Aspergillus_nidulans_PDF/6229802
<p>The proper timing and positioning of cytokinesis/septation is crucial for hyphal growth and conidiation in Aspergillus nidulans. The septation initiation network (SIN) components are a conserved spindle pole body (SPB) localized signaling cascade and the terminal kinase complex SidB-MobA, which must localize on the SPB in this pathway to trigger septation/cytokinesis. The regulatory subunit of phosphatase PP2A-ParA has been identified to be a negative regulator capable of inactivating the SIN. However, little is known about how ParA regulates the SIN pathway and whether ParA regulates the septum formation process through affecting the SPB-localized SIN proteins. In this study, through RNA-Seq and genetic approaches, we identified a new positive septation regulator, a putative mitotic-spindle organizing protein and a yeast Mzt1 homolog MztA, which acts antagonistically toward PP2A-ParA to coordinately regulate the SPB-localized SIN proteins SidB-MobA during septation. These findings imply that regulators, phosphatase PP2A-ParA and MztA counteract the septation function probably through balancing the polymerization and depolymerization of microtubules at the SPB.</p>