data_sheet_1_CD32-Expressing CD4 T Cells Are Phenotypically Diverse and Can Contain Proviral HIV DNA.docx
Genevieve E. Martin
Matthew Pace
John P. Thornhill
Chansavath Phetsouphanh
Jodi Meyerowitz
Morgane Gossez
Helen Brown
Natalia Olejniczak
Julianne Lwanga
Gita Ramjee
Pontiano Kaleebu
Kholoud Porter
Christian B. Willberg
Paul Klenerman
Nneka Nwokolo
Julie Fox
Sarah Fidler
John Frater
10.3389/fimmu.2018.00928.s001
https://frontiersin.figshare.com/articles/dataset/data_sheet_1_CD32-Expressing_CD4_T_Cells_Are_Phenotypically_Diverse_and_Can_Contain_Proviral_HIV_DNA_docx/6222428
<p>Efforts to both characterize and eradicate the HIV reservoir have been limited by the rarity of latently infected cells and the absence of a specific denoting biomarker. CD32a (FcγRIIa) has been proposed to be a marker for an enriched CD4 T cell HIV reservoir, but this finding remains controversial. Here, we explore the expression of CD32 on CD3<sup>+</sup>CD4<sup>+</sup> cells in participants from two primary HIV infection studies and identify at least three distinct phenotypes (CD32<sup>low</sup>, CD32<sup>+</sup>CD14<sup>+</sup>, and CD32<sup>high</sup>). Of note, CD4 negative enrichment kits remove the majority of CD4<sup>+</sup>CD32<sup>+</sup> T cells, potentially skewing subsequent analyses if used. CD32<sup>high</sup> CD4 T cells had higher levels of HLA-DR and HIV co-receptor expression than other subsets, compatible with their being more susceptible to infection. Surprisingly, they also expressed high levels of CD20, TCRαβ, IgD, and IgM (but not IgG), markers for both T cells and naïve B cells. Compared with other populations, CD32<sup>low</sup> cells had a more differentiated memory phenotype and high levels of immune checkpoint receptors, programmed death receptor-1 (PD-1), Tim-3, and TIGIT. Within all three CD3<sup>+</sup>CD4<sup>+</sup>CD32<sup>+</sup> phenotypes, cells could be identified in infected participants, which contained HIV DNA. CD32 expression on CD4 T cells did not correlate with HIV DNA or cell-associated HIV RNA (both surrogate measures of overall reservoir size) or predict time to rebound viremia following treatment interruption, suggesting that it is not a dominant biomarker for HIV persistence. Our data suggest that while CD32<sup>+</sup> T cells can be infected with HIV, CD32 is not a specific marker of the reservoir although it might identify a population of HIV enriched cells in certain situations.</p>
2018-05-04 13:21:37
HIV
reservoir
CD32
primary HIV infection
HIV DNA