10.3389/fmicb.2018.00818.s001
Wenping Zeng
Wenping
Zeng
Jie Wang
Jie
Wang
Ying Wang
Ying
Wang
Jing Lin
Jing
Lin
Yanping Fu
Yanping
Fu
Jiatao Xie
Jiatao
Xie
Daohong Jiang
Daohong
Jiang
Tao Chen
Tao
Chen
Huiquan Liu
Huiquan
Liu
Jiasen Cheng
Jiasen
Cheng
Data_Sheet_1_Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum.pdf
Frontiers
2018
microRNAs
sexual development
perithecium-specific milRNAs
dicer-like protein
Fusarium graminearum
2018-04-26 07:48:00
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Dicer-Like_Proteins_Regulate_Sexual_Development_via_the_Biogenesis_of_Perithecium-Specific_MicroRNAs_in_a_Plant_Pathogenic_Fungus_Fusarium_graminearum_pdf/6188486
<p>Ascospores act as the primary inoculum of Fusarium graminearum, which causes the destructive disease Fusarium head blight (FHB), or scab. MicroRNAs (miRNAs) have been reported in the F. graminearum vegetative stage, and Fgdcl2 is involved in microRNA-like RNA (milRNA) biogenesis but has no major impact on vegetative growth, abiotic stress or pathogenesis. In the present study, we found that ascospore discharge was decreased in the Fgdcl1 deletion mutant, and completely blocked in the double-deletion mutant of Fgdcl1 and Fgdcl2. Besides, more immature asci were observed in the double-deletion mutant. Interestingly, the up-regulated differentially expressed genes (DEGs) common to ΔFgdcl1 and ΔFgdcl1/2 were related to ion transmembrane transporter and membrane components. The combination of small RNA and transcriptome sequencing with bioinformatics analysis predicted 143 novel milRNAs in wild-type perithecia, and 138 of these milRNAs partly or absolutely depended on Fgdcl1, while only 5 novel milRNAs were still obtained in the Fgdcl1 and Fgdcl2 double-deletion mutant. Furthermore, 117 potential target genes were predicted. Overall, Fgdcl1 and Fgdcl2 genes were partly functionally redundant in ascospore discharge and perithecium-specific milRNA generation in F. graminearum, and these perithecium-specific milRNAs play potential roles in sexual development.</p>