10.3389/fmicb.2018.00818.s001 Wenping Zeng Wenping Zeng Jie Wang Jie Wang Ying Wang Ying Wang Jing Lin Jing Lin Yanping Fu Yanping Fu Jiatao Xie Jiatao Xie Daohong Jiang Daohong Jiang Tao Chen Tao Chen Huiquan Liu Huiquan Liu Jiasen Cheng Jiasen Cheng Data_Sheet_1_Dicer-Like Proteins Regulate Sexual Development via the Biogenesis of Perithecium-Specific MicroRNAs in a Plant Pathogenic Fungus Fusarium graminearum.pdf Frontiers 2018 microRNAs sexual development perithecium-specific milRNAs dicer-like protein Fusarium graminearum 2018-04-26 07:48:00 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Dicer-Like_Proteins_Regulate_Sexual_Development_via_the_Biogenesis_of_Perithecium-Specific_MicroRNAs_in_a_Plant_Pathogenic_Fungus_Fusarium_graminearum_pdf/6188486 <p>Ascospores act as the primary inoculum of Fusarium graminearum, which causes the destructive disease Fusarium head blight (FHB), or scab. MicroRNAs (miRNAs) have been reported in the F. graminearum vegetative stage, and Fgdcl2 is involved in microRNA-like RNA (milRNA) biogenesis but has no major impact on vegetative growth, abiotic stress or pathogenesis. In the present study, we found that ascospore discharge was decreased in the Fgdcl1 deletion mutant, and completely blocked in the double-deletion mutant of Fgdcl1 and Fgdcl2. Besides, more immature asci were observed in the double-deletion mutant. Interestingly, the up-regulated differentially expressed genes (DEGs) common to ΔFgdcl1 and ΔFgdcl1/2 were related to ion transmembrane transporter and membrane components. The combination of small RNA and transcriptome sequencing with bioinformatics analysis predicted 143 novel milRNAs in wild-type perithecia, and 138 of these milRNAs partly or absolutely depended on Fgdcl1, while only 5 novel milRNAs were still obtained in the Fgdcl1 and Fgdcl2 double-deletion mutant. Furthermore, 117 potential target genes were predicted. Overall, Fgdcl1 and Fgdcl2 genes were partly functionally redundant in ascospore discharge and perithecium-specific milRNA generation in F. graminearum, and these perithecium-specific milRNAs play potential roles in sexual development.</p>