Data_Sheet_1_Two-Photon Functional Imaging of the Auditory Cortex in Behaving Mice: From Neural Networks to Single Spines.ZIP
Ruijie Li
Meng Wang
Jiwei Yao
Shanshan Liang
Xiang Liao
Mengke Yang
Jianxiong Zhang
Junan Yan
Hongbo Jia
Xiaowei Chen
Xingyi Li
10.3389/fncir.2018.00033.s001
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Two-Photon_Functional_Imaging_of_the_Auditory_Cortex_in_Behaving_Mice_From_Neural_Networks_to_Single_Spines_ZIP/6174830
<p>In vivo two-photon Ca<sup>2+</sup> imaging is a powerful tool for recording neuronal activities during perceptual tasks and has been increasingly applied to behaving animals for acute or chronic experiments. However, the auditory cortex is not easily accessible to imaging because of the abundant temporal muscles, arteries around the ears and their lateral locations. Here, we report a protocol for two-photon Ca<sup>2+</sup> imaging in the auditory cortex of head-fixed behaving mice. By using a custom-made head fixation apparatus and a head-rotated fixation procedure, we achieved two-photon imaging and in combination with targeted cell-attached recordings of auditory cortical neurons in behaving mice. Using synthetic Ca<sup>2+</sup> indicators, we recorded the Ca<sup>2+</sup> transients at multiple scales, including neuronal populations, single neurons, dendrites and single spines, in auditory cortex during behavior. Furthermore, using genetically encoded Ca<sup>2+</sup> indicators (GECIs), we monitored the neuronal dynamics over days throughout the process of associative learning. Therefore, we achieved two-photon functional imaging at multiple scales in auditory cortex of behaving mice, which extends the tool box for investigating the neural basis of audition-related behaviors.</p>
2018-04-24 04:27:30
two-photon Ca2+ imaging
auditory cortex
behaving mouse
dendritic spines
cell-attached recordings