10.3389/fpls.2018.00430.s001 Theresa M. Rottmann Theresa M. Rottmann Carolin Fritz Carolin Fritz Anja Lauter Anja Lauter Sabine Schneider Sabine Schneider Cornelia Fischer Cornelia Fischer Nina Danzberger Nina Danzberger Petra Dietrich Petra Dietrich Norbert Sauer Norbert Sauer Ruth Stadler Ruth Stadler Data_Sheet_1_Protoplast-Esculin Assay as a New Method to Assay Plant Sucrose Transporters: Characterization of AtSUC6 and AtSUC7 Sucrose Uptake Activity in Arabidopsis Col-0 Ecotype.pdf Frontiers 2018 Arabidopsis thaliana sucrose sugar transport protein pollen tubes radioactive uptake measurement esculin protoplasts Saccharomyces cerevisiae 2018-04-23 09:52:50 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Protoplast-Esculin_Assay_as_a_New_Method_to_Assay_Plant_Sucrose_Transporters_Characterization_of_AtSUC6_and_AtSUC7_Sucrose_Uptake_Activity_in_Arabidopsis_Col-0_Ecotype_pdf/6170654 <p>The best characterized function of sucrose transporters of the SUC family in plants is the uptake of sucrose into the phloem for long-distance transport of photoassimilates. This important step is usually performed by one specific SUC in every species. However, plants possess small families of several different SUCs which are less well understood. Here, we report on the characterization of AtSUC6 and AtSUC7, two members of the SUC family in Arabidopsis thaliana. Heterologous expression in yeast (Saccharomyces cerevisiae) revealed that AtSUC6<sub>Col-0</sub> is a high-affinity H<sup>+</sup>-symporter that mediates the uptake of sucrose and maltose across the plasma membrane at exceptionally low pH values. Reporter gene analyses revealed a strong expression of AtSUC6<sub>Col-0</sub> in reproductive tissues, where the protein product might contribute to sugar uptake into pollen tubes and synergid cells. A knockout of AtSUC6 did not interfere with vegetative development or reproduction, which points toward physiological redundancy of AtSUC6<sub>Col-0</sub> with other sugar transporters. Reporter gene analyses showed that AtSUC7<sub>Col-0</sub> is expressed in roots and pollen tubes and that this sink specific expression of AtSUC7<sub>Col-0</sub> is regulated by intragenic regions. Transport activity of AtSUC7<sub>Col-0</sub> could not be analyzed in baker’s yeast or Xenopus oocytes because the protein was not correctly targeted to the plasma membrane in both heterologous expression systems. Therefore, a novel approach to analyze sucrose transporters in planta was developed. Plasma membrane localized SUCs including AtSUC6<sub>Col-0</sub> and also sucrose specific SWEETs were able to mediate transport of the fluorescent sucrose analog esculin in transformed mesophyll protoplasts. In contrast, AtSUC7<sub>Col-0</sub> is not able to mediate esculin transport across the plasma membrane which implicates that AtSUC7<sub>Col-0</sub> might be a non-functional pseudogene. The novel protoplast assay provides a useful tool for the quick and quantitative analysis of sucrose transporters in an in planta expression system.</p>