Hyvärinen, Kati Holopainen, Minna Skirdenko, Vita Ruhanen, Hanna Lehenkari, Petri Korhonen, Matti Käkelä, Reijo Laitinen, Saara Kerkelä, Erja Data_Sheet_1.docx <p>Resolution-phase macrophage population orchestrates active dampening of the inflammation by secreting anti-inflammatory and proresolving products including interleukin (IL)-10 and lipid mediators (LMs). We investigated the effects of both human bone marrow-derived mesenchymal stromal cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) on mature human regulatory macrophages (Mregs). The cytokines and LMs were determined from cell culture media of Mregs cultivated with MSCs and MSC-EVs. In addition, the alterations in the expression of cell surface markers and the phagocytic ability of Mregs were investigated. Our novel findings indicate that both MSC coculture and MSC-EVs downregulated the production of IL-23 and IL-22 enhancing the anti-inflammatory phenotype of Mregs and amplifying proresolving properties. The levels of prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) were substantially upregulated in MSC coculture media, which may endorse proresolving LM class switching. In addition, our results manifest, for the first time, that MSC-EVs mediate the Mreg phenotype change via PGE<sub>2</sub>. These data suggest that both human MSC and MSC-EVs may potentiate tolerance-promoting proresolving phenotype of human Mregs.</p> regulatory macrophages;mesenchymal stromal cells;extracellular vesicles;interleukin-23;prostaglandin E2;resolution 2018-04-12
    https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_docx/6131519
10.3389/fimmu.2018.00771.s001