10.3389/fmicb.2018.00674.s001 Késia S. Lourenço Késia S. Lourenço Noriko A. Cassman Noriko A. Cassman Agata S. Pijl Agata S. Pijl Johannes A. van Veen Johannes A. van Veen Heitor Cantarella Heitor Cantarella Eiko E. Kuramae Eiko E. Kuramae Data_Sheet_1.docx Frontiers 2018 nitrogen and carbon biochemical cycles recycling vinasse quantitative real time PCR amoA gene sugarcane 2018-04-10 04:04:31 Dataset https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_docx/6119552 <p>Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (N<sub>2</sub>O) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the N<sub>2</sub>O emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and N<sub>2</sub>O emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene (amoA) encoding the ammonia monooxygenase enzyme, bacterial denitrifier (nirK, nirS, and nosZ) genes and total bacteria were quantified by real time PCR. We also employed a deep amoA amplicon sequencing approach to evaluate the effect of treatment on the community structure and diversity of the soil AOB community. Both vinasse types applied with inorganic N application increased the total N<sub>2</sub>O emissions and the abundance of AOB. Nitrosospira sp. was the dominant AOB in the soil and was correlated with N<sub>2</sub>O emissions. However, the diversity and the community structure of AOB did not change with vinasse and inorganic N fertilizer amendment. The results highlight the importance of residues and fertilizer management in sustainable agriculture and can be used as a reference and an input tool to determine good management practices for organic fertilization.</p>