10.3389/fmicb.2018.00674.s001
Késia S. Lourenço
Késia S.
Lourenço
Noriko A. Cassman
Noriko A.
Cassman
Agata S. Pijl
Agata
S. Pijl
Johannes A. van Veen
Johannes
A. van Veen
Heitor Cantarella
Heitor
Cantarella
Eiko E. Kuramae
Eiko
E. Kuramae
Data_Sheet_1.docx
Frontiers
2018
nitrogen and carbon biochemical cycles
recycling
vinasse
quantitative real time PCR
amoA gene
sugarcane
2018-04-10 04:04:31
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_docx/6119552
<p>Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (N<sub>2</sub>O) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the N<sub>2</sub>O emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and N<sub>2</sub>O emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene (amoA) encoding the ammonia monooxygenase enzyme, bacterial denitrifier (nirK, nirS, and nosZ) genes and total bacteria were quantified by real time PCR. We also employed a deep amoA amplicon sequencing approach to evaluate the effect of treatment on the community structure and diversity of the soil AOB community. Both vinasse types applied with inorganic N application increased the total N<sub>2</sub>O emissions and the abundance of AOB. Nitrosospira sp. was the dominant AOB in the soil and was correlated with N<sub>2</sub>O emissions. However, the diversity and the community structure of AOB did not change with vinasse and inorganic N fertilizer amendment. The results highlight the importance of residues and fertilizer management in sustainable agriculture and can be used as a reference and an input tool to determine good management practices for organic fertilization.</p>