Image_2.PDF
Mengfei Song
Qingzhen Wei
Jing Wang
Wenyuan Fu
Xiaodong Qin
Xiumei Lu
Feng Cheng
Kang Yang
Lu Zhang
Xiaqing Yu
Ji Li
Jinfeng Chen
Qunfeng Lou
10.3389/fpls.2018.00432.s002
https://frontiersin.figshare.com/articles/figure/Image_2_PDF/6106028
<p>Leaf color mutants in higher plants are ideal materials for investigating the structure and function of photosynthetic system. In this study, we identified a cucumber vyl (virescent-yellow leaf) mutant in the mutant library, which exhibited reduced pigment contents and delayed chloroplast development process. F<sub>2</sub> and BC<sub>1</sub> populations were constructed from the cross between vyl mutant and cucumber inbred line ‘Hazerd’ to identify that the vyl trait is controlled by a simply recessive gene designated as CsVYL. The CsVYL gene was mapped to a 3.8 cM interval on chromosome 4 using these 80 F<sub>2</sub> individuals and BSA (bulked segregation analysis) approach. Fine genetic map was conducted with 1542 F<sub>2</sub> plants and narrowed down the vyl locus to an 86.3 kb genomic region, which contains a total of 11 genes. Sequence alignment between the wild type (WT) and vyl only identified one single nucleotide mutation (C→T) in the first exon of gene Csa4G637110, which encodes a DnaJ-like zinc finger protein. Gene Expression analysis confirmed the differences in transcription level of Csa4G637110 between wild type and mutant plants. Map-based cloning of the CsVYL gene could accelerate the study of chloroplast development and chlorophyll synthesis of cucumber.</p>
2018-04-06 08:29:01
Cucumis sativus L.
leaf color mutant
virescent
chloroplast function
DnaJ proteins