10.3389/fchem.2018.00094.s004 Boshi Liu Boshi Liu Renliang Huang Renliang Huang Yanjun Yu Yanjun Yu Rongxin Su Rongxin Su Wei Qi Wei Qi Zhimin He Zhimin He Image3.PDF Frontiers 2018 aptamer LSPR ochratoxin A AuNP aggregation colorimetric detection 2018-04-04 04:43:06 Figure https://frontiersin.figshare.com/articles/figure/Image3_PDF/6085493 <p>Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.</p>