10.3389/fchem.2018.00094.s004
Boshi Liu
Boshi
Liu
Renliang Huang
Renliang
Huang
Yanjun Yu
Yanjun
Yu
Rongxin Su
Rongxin
Su
Wei Qi
Wei
Qi
Zhimin He
Zhimin
He
Image3.PDF
Frontiers
2018
aptamer
LSPR
ochratoxin A
AuNP aggregation
colorimetric detection
2018-04-04 04:43:06
Figure
https://frontiersin.figshare.com/articles/figure/Image3_PDF/6085493
<p>Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.</p>