10.3389/fnmol.2018.00090.s002
Charlotte Benoit
Charlotte
Benoit
Soraya Doubi-Kadmiri
Soraya
Doubi-Kadmiri
Xavier Benigni
Xavier
Benigni
Delphine Crepin
Delphine
Crepin
Laure Riffault
Laure
Riffault
Ghislaine Poizat
Ghislaine
Poizat
Claire-Marie Vacher
Claire-Marie
Vacher
Mohammed Taouis
Mohammed
Taouis
Anne Baroin-Tourancheau
Anne
Baroin-Tourancheau
Laurence Amar
Laurence
Amar
Table2.xls
Frontiers
2018
aging
brain
Illumina sequencing
metabolic environment
metabolic programming
miRNA expression
miRNome
2018-03-28 04:21:29
Dataset
https://frontiersin.figshare.com/articles/dataset/Table2_xls/6045659
<p>Epidemiological reports and studies using rodent models indicate that early exposure to nutrient and/or hormonal challenges can reprogram metabolism at adulthood. Hypothalamic arcuate nucleus (ARC) integrates peripheral and central signals to adequately regulate energy homeostasis. microRNAs (miRNAs) participate in the control of gene expression of large regulatory networks including many signaling pathways involved in epigenetics regulations. Here, we have characterized and compared the miRNA population of ARC of adult male rats continuously exposed to a balanced metabolic environment to the one of adult male rats exposed to an unbalanced high-fat/high-carbohydrate/moderate-protein metabolic environment during the perinatal period and/or at adulthood that consequently displayed hyperinsulinemia and/or hyperleptinemia. We identified more than 400 miRNA species in ARC of adult male rats. By comparing the miRNA content of six biological replicates in each of the four perinatal/adult environments/rat groups, we identified the 10 miRNAs specified by clusters miR-96/182/183, miR-141/200c, and miR-200a/200b/429 as miRNAs of systematic and uncommonly high variation of expression. This uncommon variation of expression may underlie high individual differences in aging disease susceptibilities. By comparing the miRNA content of the adult ARC between the rat groups, we showed that the miRNA population was not affected by the unbalanced adult environment while, in contrast, the expression of 11 miRNAs was repeatedly impacted by the perinatal unbalanced environment. Our data revealed a miRNA response of adult ARC to early metabolic environmental challenge.</p>