10.3389/fcimb.2018.00087.s005
Jonathan Ferrand
Jonathan
Ferrand
Nathan P. Croft
Nathan
P. Croft
Geneviève Pépin
Geneviève
Pépin
Kerrilyn R. Diener
Kerrilyn
R. Diener
Di Wu
Di
Wu
Niamh E. Mangan
Niamh E.
Mangan
John Pedersen
John
Pedersen
Mark A. Behlke
Mark A.
Behlke
John D. Hayball
John D.
Hayball
Anthony W. Purcell
Anthony W.
Purcell
Richard L. Ferrero
Richard
L. Ferrero
Michael P. Gantier
Michael
P. Gantier
Table5.XLSX
Frontiers
2018
Salmonella
CRISPR/CAS9
congenic mice
background contamination
host-pathogen interactions
2018-03-19 04:09:18
Dataset
https://frontiersin.figshare.com/articles/dataset/Table5_XLSX/5998703
<p>Murine models of Salmonella enterica serovar Typhimurium infection are one of the commonest tools to study host-pathogen interactions during bacterial infections. Critically, the outcome of S. Typhimurium infection is impacted by the genetic background of the mouse strain used, with macrophages from C57BL/6 and BALB/c mice lacking the capacity to control intracellular bacterial replication. For this reason, the use of congenic strains, which mix the genetic backgrounds of naturally protected mouse strains with those of susceptible strains, has the capacity to significantly alter results and interpretation of S. Typhimurium infection studies. Here, we describe how macrophage knockout cell lines generated by CRISPR/Cas9 gene editing can help determine the contribution of background contaminations in the phenotypes of primary macrophages from congenic mice, on the outcome of S. Typhimurium infection studies. Our own experience illustrates how the CRISPR/Cas9 technology can be used to complement pre-existing knockout models, and shows that there is great merit in performing concurrent studies with both genetic models, to exclude unanticipated side-effects on host-pathogen interactions.</p>