DataSheet1.DOCX
Pol Alonso-Pernas
Stefan Bartram
Erika M. Arias-Cordero
Alexey L. Novoselov
Lorena Halty-deLeon
Yongqi Shao
Wilhelm Boland
10.3389/fmicb.2017.01970.s001
https://frontiersin.figshare.com/articles/dataset/DataSheet1_DOCX/5972098
<p>The guts of insects harbor symbiotic bacterial communities. However, due to their complexity, it is challenging to relate a specific symbiotic phylotype to its corresponding function. In the present study, we focused on the forest cockchafer (Melolontha hippocastani), a phytophagous insect with a dual life cycle, consisting of a root-feeding larval stage and a leaf-feeding adult stage. By combining in vivo stable isotope probing (SIP) with <sup>13</sup>C cellulose and <sup>15</sup>N urea as trophic links, with Illumina MiSeq (Illumina-SIP), we unraveled bacterial networks processing recalcitrant dietary components and recycling nitrogenous waste. The bacterial communities behind these processes change between larval and adult stages. In <sup>13</sup>C cellulose-fed insects, the bacterial families Lachnospiraceae and Enterobacteriaceae were isotopically labeled in larvae and adults, respectively. In <sup>15</sup>N urea-fed insects, the genera Burkholderia and Parabacteroides were isotopically labeled in larvae and adults, respectively. Additionally, the PICRUSt-predicted metagenome suggested a possible ability to degrade hemicellulose and to produce amino acids of, respectively, <sup>13</sup>C cellulose- and <sup>15</sup>N urea labeled bacteria. The incorporation of <sup>15</sup>N from ingested urea back into the insect body was confirmed, in larvae and adults, by isotope ratio mass spectrometry (IRMS). Besides highlighting key bacterial symbionts of the gut of M. hippocastani, this study provides example on how Illumina-SIP with multiple trophic links can be used to target microorganisms embracing different roles within an environment.</p>
2018-03-12 08:13:21
Melolontha hippocastani
nitrogen recycling
cellulose degradation
gut bacteria
symbiotic bacteria
Illumina-SIP
IRMS