10.3389/fchem.2018.00034.s001
Jayanna N. Banavath
Jayanna N.
Banavath
Thammineni Chakradhar
Thammineni
Chakradhar
Varakumar Pandit
Varakumar
Pandit
Sravani Konduru
Sravani
Konduru
Krishna K. Guduru
Krishna K.
Guduru
Chandra S. Akila
Chandra S.
Akila
Sudhakar Podha
Sudhakar
Podha
Chandra O. R. Puli
Chandra O. R.
Puli
DataSheet1.DOC
Frontiers
2018
peanut
AtHDG11 (Arabidopsis Homeodomain globarous11)
drought stress
high-salinity stress
water use efficiency
yield potential
2018-03-02 04:26:59
Dataset
https://frontiersin.figshare.com/articles/dataset/DataSheet1_DOC/5942797
<p>Peanut is an important oilseed and food legume cultivated as a rain-fed crop in semi-arid tropics. Drought and high salinity are the major abiotic stresses limiting the peanut productivity in this region. Development of drought and salt tolerant peanut varieties with improved yield potential using biotechnological approach is highly desirable to improve the peanut productivity in marginal geographies. As abiotic stress tolerance and yield represent complex traits, engineering of regulatory genes to produce abiotic stress-resilient transgenic crops appears to be a viable approach. In the present study, we developed transgenic peanut plants expressing an Arabidopsis homeodomain-leucine zipper transcription factor (AtHDG11) under stress inducible rd29A promoter. A stress-inducible expression of AtHDG11 in three independent homozygous transgenic peanut lines resulted in improved drought and salt tolerance through up-regulation of known stress responsive genes (LEA, HSP70, Cu/Zn SOD, APX, P5CS, NCED1, RRS5, ERF1, NAC4, MIPS, Aquaporin, TIP, ELIP) in the stress gene network, antioxidative enzymes, free proline along with improved water use efficiency traits such as longer root system, reduced stomatal density, higher chlorophyll content, increased specific leaf area, improved photosynthetic rates, and increased intrinsic instantaneous WUE. Transgenic peanut plants displayed high yield compared to non-transgenic plants under both drought and salt stress conditions. Holistically, our study demonstrates the potentiality of stress-induced expression of AtHDG11 to improve the drought, salt tolerance in peanut.</p>