10.3389/fimmu.2018.00360.s002 Hanaa Safya Hanaa Safya Amine Mellouk Amine Mellouk Julie Legrand Julie Legrand Sylvain M. Le Gall Sylvain M. Le Gall Mohcine Benbijja Mohcine Benbijja Colette Kanellopoulos-Langevin Colette Kanellopoulos-Langevin Jean M. Kanellopoulos Jean M. Kanellopoulos Pierre Bobé Pierre Bobé Image_2.tif Frontiers 2018 P2X7 CD39 CD73 regulatory T lymphocyte CD62L shedding pore formation phosphatidyslerine exposure cell death 2018-02-27 04:32:30 Figure https://frontiersin.figshare.com/articles/figure/Image_2_tif/5926927 <p>A previous report has shown that regulatory T cells (Treg) were markedly more sensitive to adenosine-5′-triphosphate (ATP) than conventional T cells (Tconv). Another one has shown that Tregs and CD45RB<sup>low</sup> Tconvs, but not CD45RB<sup>high</sup> Tconvs, displayed similar high sensitivity to ATP. We have previously reported that CD45RB<sup>low</sup> Tconvs expressing B220/CD45RABC molecules in a pre-apoptotic stage are resistant to ATP stimulation due to the loss of P2X7 receptor (P2X7R) membrane expression. To gain a clearer picture on T-cell sensitivity to ATP, we have quantified four different cellular activities triggered by ATP in mouse T cells at different stages of activation/differentiation, in correlation with levels of P2X7R membrane expression. P2X7R expression significantly increases on Tconvs during differentiation from naive CD45RB<sup>high</sup>CD44<sup>low</sup> to effector/memory CD45RB<sup>low</sup>CD44<sup>high</sup> stage. Maximum levels of upregulation are reached on recently activated CD69<sup>+</sup> naive and memory Tconvs. Ectonucleotidases CD39 and CD73 expression levels increase in parallel with those of P2X7R. Recently activated CD69<sup>+</sup> CD45RB<sup>high</sup>CD44<sup>low</sup> Tconvs, although expressing high levels of P2X7R, fail to cleave homing receptor CD62L after ATP treatment, but efficiently form pores and externalize phosphatidylserine (PS). In contrast, naive CD45RB<sup>high</sup>CD44<sup>low</sup> Tconvs cleave CD62L with high efficiency although they express a lower level of P2X7, thus suggesting that P2X7R levels are not a limiting factor for signaling ATP-induced cellular responses. Contrary to common assumption, P2X7R-mediated cellular activities in mouse Tconvs are not triggered in an all-or-none manner, but depend on their stage of activation/differentiation. Compared to CD45RB<sup>low</sup> Tconvs, CD45RB<sup>low</sup>Foxp3<sup>+</sup> Tregs show significantly higher levels of P2X7R membrane expression and of sensitivity to ATP as evidenced by their high levels of CD62L shedding, pore formation and PS externalization observed after ATP treatment. In summary, the different abilities of ATP-treated Tconvs to form pore or cleave CD62L depending on their activation and differentiation state suggests that P2X7R signaling varies according to the physiological role of T convs during antigen activation in secondary lymphoid organs or trafficking to inflammatory sites.</p>