10.3389/fmicb.2018.00277.s004
Carmen Gómez-Lama Cabanás
Carmen Gómez-Lama
Cabanás
Garikoitz Legarda
Garikoitz
Legarda
David Ruano-Rosa
David
Ruano-Rosa
Paloma Pizarro-Tobías
Paloma
Pizarro-Tobías
Antonio Valverde-Corredor
Antonio
Valverde-Corredor
José L. Niqui
José L.
Niqui
Juan C. Triviño
Juan C.
Triviño
Amalia Roca
Amalia
Roca
Jesús Mercado-Blanco
Jesús
Mercado-Blanco
DataSheet4.XLSX
Frontiers
2018
Olea europaea
verticillium wilt
biocontrol
Pseudomonas
rhizobacteria
Pseudomonas indica
2018-02-23 04:26:43
Dataset
https://frontiersin.figshare.com/articles/dataset/DataSheet4_XLSX/5917735
<p>The use of biological control agents (BCA), alone or in combination with other management measures, has gained attention over the past decades, driven by the need to seek for sustainable and eco-friendly alternatives to confront plant pathogens. The rhizosphere of olive (Olea europaea L.) plants is a source of bacteria with potential as biocontrol tools against Verticillium wilt of olive (VWO) caused by Verticillium dahliae Kleb. A collection of bacterial isolates from healthy nursery-produced olive (cultivar Picual, susceptible to VWO) plants was generated based on morphological, biochemical and metabolic characteristics, chemical sensitivities, and on their in vitro antagonistic activity against several olive pathogens. Three strains (PIC25, PIC105, and PICF141) showing high in vitro inhibition ability of pathogens' growth, particularly against V. dahliae, were eventually selected. Their effectiveness against VWO caused by the defoliating pathotype of V. dahliae was also demonstrated, strain PICF141 being the rhizobacteria showing the best performance as BCA. Genotypic and phenotypic traits traditionally associated with plant growth promotion and/or biocontrol abilities were evaluated as well (e.g., phytase, xylanase, catalase, cellulase, chitinase, glucanase activities, and siderophore and HCN production). Multi-locus sequence analyses of conserved genes enabled the identification of these strains as Pseudomonas spp. Strain PICF141 was affiliated to the “Pseudomonas mandelii subgroup,” within the “Pseudomonas fluorescens group,” Pseudomonas lini being the closest species. Strains PIC25 and PIC105 were affiliated to the “Pseudomonas aeruginosa group,” Pseudomonas indica being the closest relative. Moreover, we identified P. indica (PIC105) for the first time as a BCA. Genome sequencing and in silico analyses allowed the identification of traits commonly associated with plant-bacteria interactions. Finally, the root colonization ability of these olive rhizobacteria was assessed, providing valuable information for the future development of formulations based on these strains. A set of actions, from rhizosphere isolation to genome analysis, is proposed and discussed for selecting indigenous rhizobacteria as effective BCAs.</p>