10.3389/fncel.2018.00041.s004
Clive P. Morgan
Clive P.
Morgan
Hongyu Zhao
Hongyu
Zhao
Meredith LeMasurier
Meredith
LeMasurier
Wei Xiong
Wei
Xiong
Bifeng Pan
Bifeng
Pan
Piotr Kazmierczak
Piotr
Kazmierczak
Matthew R. Avenarius
Matthew
R. Avenarius
Michael Bateschell
Michael
Bateschell
Ruby Larisch
Ruby
Larisch
Anthony J. Ricci
Anthony
J. Ricci
Ulrich Müller
Ulrich
Müller
Peter G. Barr-Gillespie
Peter G.
Barr-Gillespie
Image_4.tif
Frontiers
2018
hair cells
stereocilia
mechanotransduction
TRP channels
auditory brainstem response (ABR)
2018-02-20 04:34:40
Figure
https://frontiersin.figshare.com/articles/figure/Image_4_tif/5902627
<p>Hair cells of the inner ear transduce mechanical stimuli like sound or head movements into electrical signals, which are propagated to the central nervous system. The hair-cell mechanotransduction channel remains unidentified. We tested whether three transient receptor channel (TRP) family members, TRPV6, TRPM6 and TRPM7, were necessary for transduction. TRPV6 interacted with USH1C (harmonin), a scaffolding protein that participates in transduction. Using a cysteine-substitution knock-in mouse line and methanethiosulfonate (MTS) reagents selective for this allele, we found that inhibition of TRPV6 had no effect on transduction in mouse cochlear hair cells. TRPM6 and TRPM7 each interacted with the tip-link component PCDH15 in cultured eukaryotic cells, which suggested they might be part of the transduction complex. Cochlear hair cell transduction was not affected by manipulations of Mg<sup>2+</sup>, however, which normally perturbs TRPM6 and TRPM7. To definitively examine the role of these two channels in transduction, we showed that deletion of either or both of their genes selectively in hair cells had no effect on auditory function. We suggest that TRPV6, TRPM6 and TRPM7 are unlikely to be the pore-forming subunit of the hair-cell transduction channel.</p>