10.3389/fimmu.2018.00291.s001
Kostas Patas
Kostas
Patas
Anne Willing
Anne
Willing
Cüneyt Demiralay
Cüneyt
Demiralay
Jan Broder Engler
Jan Broder
Engler
Andreea Lupu
Andreea
Lupu
Caren Ramien
Caren
Ramien
Tobias Schäfer
Tobias
Schäfer
Christian Gach
Christian
Gach
Laura Stumm
Laura
Stumm
Kenneth Chan
Kenneth
Chan
Marissa Vignali
Marissa
Vignali
Petra C. Arck
Petra C.
Arck
Manuel A. Friese
Manuel A.
Friese
Ole Pless
Ole
Pless
Klaus Wiedemann
Klaus
Wiedemann
Agorastos Agorastos
Agorastos
Agorastos
Stefan M. Gold
Stefan M.
Gold
Data_Sheet_1.PDF
Frontiers
2018
adaptive immunity
major depressive disorder
chemokine receptors
regulatory T cells
T cell receptor repertoire
2018-02-20 04:23:22
Dataset
https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_PDF/5902561
<p>While a link between inflammation and the development of neuropsychiatric disorders, including major depressive disorder (MDD) is supported by a growing body of evidence, little is known about the contribution of aberrant adaptive immunity in this context. Here, we conducted in-depth characterization of T cell phenotype and T cell receptor (TCR) repertoire in MDD. For this cross-sectional case–control study, we recruited antidepressant-free patients with MDD without any somatic or psychiatric comorbidities (n = 20), who were individually matched for sex, age, body mass index, and smoking status to a non-depressed control subject (n = 20). T cell phenotype and repertoire were interrogated using a combination of flow cytometry, gene expression analysis, and next generation sequencing. T cells from MDD patients showed significantly lower surface expression of the chemokine receptors CXCR3 and CCR6, which are known to be central to T cell differentiation and trafficking. In addition, we observed a shift within the CD4<sup>+</sup> T cell compartment characterized by a higher frequency of CD4<sup>+</sup>CD25<sup>high</sup>CD127<sup>low/−</sup> cells and higher FOXP3 mRNA expression in purified CD4<sup>+</sup> T cells obtained from patients with MDD. Finally, flow cytometry-based TCR Vβ repertoire analysis indicated a less diverse CD4<sup>+</sup> T cell repertoire in MDD, which was corroborated by next generation sequencing of the TCR β chain CDR3 region. Overall, these results suggest that T cell phenotype and TCR utilization are skewed on several levels in patients with MDD. Our study identifies putative cellular and molecular signatures of dysregulated adaptive immunity and reinforces the notion that T cells are a pathophysiologically relevant cell population in this disorder.</p>