10.3389/fpls.2020.01142.s004
Shaoyu Zhang
Shaoyu
Zhang
Guangyu Ding
Guangyu
Ding
Wenmin He
Wenmin
He
Kai Liu
Kai
Liu
Yiwei Luo
Yiwei
Luo
Jiaqi Tang
Jiaqi
Tang
Ningjia He
Ningjia
He
Table_2_Functional Characterization of the 1-Deoxy-D-Xylulose 5-Phosphate Synthase Genes in Morus notabilis.docx
Frontiers
2020
terpenoids
1-deoxy-D-xylulose 5-phosphate synthase
Morus notabilis
functional differentiation
early flowering
2020-07-24 13:50:14
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_2_Functional_Characterization_of_the_1-Deoxy-D-Xylulose_5-Phosphate_Synthase_Genes_in_Morus_notabilis_docx/12708602
<p>Terpenoids are considered to be the largest group of secondary metabolites and natural products. Studies have revealed 1-deoxy-D-xylulose 5-phosphate synthase (DXS) is the first and rate-limiting enzyme in the plastidial methylerythritol phosphate pathway, which produces isopentenyl diphosphate and its isoform dimethylallyl diphosphate as terpenoid biosynthesis precursors. Mulberry (Morus L.) is an economically and ecologically important perennial tree with diverse secondary metabolites, including terpenoids that protect plants against bacteria and insects and may be useful for treating human diseases. However, there has been relatively little research regarding DXS genes in mulberry and other woody plant species. In this study, we cloned and functionally characterized three Morus notabilis DXS genes (MnDXS1, MnDXS2A, and MnDXS2B). Bioinformatics analyses indicated MnDXS1 belongs to clade 1, whereas MnDXS2A and MnDXS2B are in clade 2. The three encoded MnDXS proteins are localized to chloroplasts. Additionally, substantial differences in MnDXS expression patterns were observed in diverse tissues and in response to insect feeding and methyl jasmonate treatment. Moreover, overexpression of MnDXS1 in Arabidopsis thaliana increased the gibberellic acid content and resulted in early flowering, whereas overexpression of MnDXS2A enhanced root growth and increased the chlorophyll and carotenoid content. Our findings indicate that MnDXS functions vary among the clades, which may be useful for further elucidation of the functions of the DXS genes in mulberry.</p>