10.3389/fncel.2020.00199.s001
Xiao Luo
Xiao
Luo
Ting He
Ting
He
Yan Wang
Yan
Wang
Jiang-Lin Wang
Jiang-Lin
Wang
Xue-Bin Yan
Xue-Bin
Yan
Hao-Cheng Zhou
Hao-Cheng
Zhou
Rui-Rui Wang
Rui-Rui
Wang
Rui Du
Rui
Du
Xiao-Liang Wang
Xiao-Liang
Wang
Jun Chen
Jun
Chen
Dong Huang
Dong
Huang
Table_1_Ceftriaxone Relieves Trigeminal Neuropathic Pain Through Suppression of Spatiotemporal Synaptic Plasticity via Restoration of Glutamate Transporter 1 in the Medullary Dorsal Horn.DOC
Frontiers
2020
trigeminal neuropathic pain
glutamate transporter 1
ceftriaxone
synaptic plasticity
long-term potentiation
medullary dorsal horn
2020-06-30 14:48:42
Dataset
https://frontiersin.figshare.com/articles/dataset/Table_1_Ceftriaxone_Relieves_Trigeminal_Neuropathic_Pain_Through_Suppression_of_Spatiotemporal_Synaptic_Plasticity_via_Restoration_of_Glutamate_Transporter_1_in_the_Medullary_Dorsal_Horn_DOC/12589124
<p>Using a rat model of trigeminal neuropathic pain (TNP) produced by chronic compression of the infraorbital nerve (CCI-ION), we investigated the analgesic effect and the underlying mechanisms of ceftriaxone (Cef), a β-lactam antibiotic, that is thought to be a potent stimulator of glutamate transporter 1 (GLT-1). First, repeated intraperitoneal (i.p.) injections of Cef (200 mg/kg) for 5-days since Day 1 of CCI-ION could significantly relieve both mechanical and thermal pain hypersensitivity from day 10 after drug administration. Western blot and immunofluorescent results demonstrated that 5-days administration of Cef resulted in the restoration of GLT-1 expression to a level equivalent to the sham control which was dramatically lost under the TNP condition. Moreover, multi-electrode (8 × 8) array recordings of network field excitatory postsynaptic potentials (fEPSPs) were performed on the acutely dissociated medullary dorsal horn slice evoked by electrical stimulation of the trigeminal spinal tract. The results showed that the increased number of fEPSPs, induction rate, and maintenance of long-term potentiation caused by CCI-ION were significantly suppressed by 5-days administration of Cef. Taken together, the results indicate that Cef can relieve TNP through suppression of spatiotemporal synaptic plasticity via GLT-1 restoration in the medullary dorsal horn of the trigeminal nerve.</p>